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mmu_circAdcy1_381
Basic information of circular RNA
PanCircBase ID:
mmu_circAdcy1_381
mm39_Chr. Position ID:
chr11|7028887|7059112|+
Gene name:
Adcy1
Transcript:
ENSMUST00000020706.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,21682,30113
Genomic length:
30225
Exon/intron sizes in nt:
150,119,112
Flanking exon/introns:
chr11:7014235-7028887|chr11:7059112-7080275
Splice length:
381
Other database IDs:
circBase: mmu_circ_0000232, CIRCpedia: MMU_CIRCpedia_27613, circAtlas: mmu-Adcy1_0001
circBase: hsa_circ_0134390, circAtlas: hsa-ADCY1_0002, CIRCpedia: HSA_CIRCpedia_145072
Human Ortholog IDs:
Mature spliced Sequence:
CTCGGTGCCAATGCCCTGCTCTTCTTTGGTGTGAACATGTATGGCGTCTTCGTGCGGATCTTGACAGAGCGCTCCCAGAGGAAGGCCTTCCTGCAGGCTCGAAACTGCATTGAGGACCGGCTGCGGCTGGAGGATGAGAATGAGAAGCAGGAACGGTTGCTCATGAGCCTCTTGCCCCGGAATGTCGCCATGGAGATGAAGGAAGACTTCCTGAAGCCCCCTGAGAGGATTTTTCACAAGATTTACATCCAGAGGCATGACAATGTGAGCATCCTGTTTGCAGACATCGTGGGTTTCACAGGTCTGGCGTCCCAGTGCACAGCCCAGGAGCTGGTGAAACTGCTCAATGAACTCTTTGGCAAGTTCGATGAGTTAGCCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGTGGGTTTCACAGGTCTG
59.89
CTCTGTCAAGATCCGCACGA
59.83
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGTGGGTTTCACAGGTCTG
59.89
GCACGAAGACGCCATACATG
59.7
150
PCR template sequence:
AGACATCGTGGGTTTCACAGGTCTGGCGTCCCAGTGCACAGCCCAGGAGCTGGTGAAACTGCTCAATGAACTCTTTGGCAAGTTCGATGAGTTAGCCACGCTCGGTGCCAATGCCCTGCTCTTCTTTGGTGTGAACATGTATGGCGTCTTCGTGCGGATCTTGACAGAGCGCTCCCAGAGGAAGGCCTTCCTGCAGGCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-31-5p(1), mmu-miR-205-5p(1), mmu-miR-23a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGAGTTAGCCACGCTCGGTGCCAATG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADCY1_381_ORF_1
1.2611
0.57266689
0.990665959
726
ORF sequence:
ATGTATGGCGTCTTCGTGCGGATCTTGACAGAGCGCTCCCAGAGGAAGGCCTTCCTGCAGGCTCGAAACTGCATTGAGGACCGGCTGCGGCTGGAGGATGAGAATGAGAAGCAGGAACGGTTGCTCATGAGCCTCTTGCCCCGGAATGTCGCCATGGAGATGAAGGAAGACTTCCTGAAGCCCCCTGAGAGGATTTTTCACAAGATTTACATCCAGAGGCATGACAATGTGAGCATCCTGTTTGCAGACATCGTGGGTTTCACAGGTCTGGCGTCCCAGTGCACAGCCCAGGAGCTGGTGAAACTGCTCAATGAACTCTTTGGCAAGTTCGATGAGTTAGCCACGCTCGGTGCCAATGCCCTGCTCTTCTTTGGTGTGAACATGTATGGCGTCTTCGTGCGGATCTTGACAGAGCGCTCCCAGAGGAAGGCCTTCCTGCAGGCTCGAAACTGCATTGAGGACCGGCTGCGGCTGGAGGATGAGAATGAGAAGCAGGAACGGTTGCTCATGAGCCTCTTGCCCCGGAATGTCGCCATGGAGATGAAGGAAGACTTCCTGAAGCCCCCTGAGAGGATTTTTCACAAGATTTACATCCAGAGGCATGACAATGTGAGCATCCTGTTTGCAGACATCGTGGGTTTCACAGGTCTGGCGTCCCAGTGCACAGCCCAGGAGCTGGTGAAACTGCTCAATGAACTCTTTGGCAAGTTCGATGAGTTAGCCACG
Peptide sequence:
MYGVFVRILTERSQRKAFLQARNCIEDRLRLEDENEKQERLLMSLLPRNVAMEMKEDFLKPPERIFHKIYIQRHDNVSILFADIVGFTGLASQCTAQELVKLLNELFGKFDELATLGANALLFFGVNMYGVFVRILTERSQRKAFLQARNCIEDRLRLEDENEKQERLLMSLLPRNVAMEMKEDFLKPPERIFHKIYIQRHDNVSILFADIVGFTGLASQCTAQELVKLLNELFGKFDELAT
IRESfinder result
IRESfinder score
0.252732916
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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