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mmu_circAdck1_318
Basic information of circular RNA
PanCircBase ID:
mmu_circAdck1_318
mm39_Chr. Position ID:
chr12|88397790|88408011|+
Gene name:
Adck1
Transcript:
ENSMUST00000101165.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,6
Exon/intron offsets:
0,10062
Genomic length:
10221
Exon/intron sizes in nt:
1,59,159
Flanking exon/introns:
chr12:88368946-88397790|chr12:88408011-88413410
Splice length:
318
Other database IDs:
CIRCpedia: MMU_CIRCpedia_224337, circAtlas: mmu-Adck1_0003
circAtlas: hsa-ADCK1_0030
Human Ortholog IDs:
Mature spliced Sequence:
ATCCACGATTTGTTCCTGAGCTTCGATGACACCCCTCTTGGGGCAGCCTCCCTGGCCCAGGTCCACAAGGCGGTGTTGCATGATGGTCGGACAGTGGCAGTTAAGGTCCAGCACCCGAAGGTTCAGGCTCAAAGCTCTAAGGACATTCTCCTGATGGAGGTGCTTGTCCTGGCCGTGAAGCAACTTTTCCCAGATTTTGAATTCATGTGGCTGGTGGATGAAGCGAAGAAGAACCTGCCTCTCGAGTTGGACTTCCTGAATGAAGGGAGGAACGCTGAGAAAGTGGCCCACATGCTCAGGCACTTTGACTTCCTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTCTCGAGTTGGACTTCC
60.11
TGTCCGACCATCATGCAACA
59.96
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAGGAACGCTGAGAAAGT
59.68
TGTCCGACCATCATGCAACA
59.96
147
PCR template sequence:
TGAAGCGAAGAAGAACCTGCCTCTCGAGTTGGACTTCCTGAATGAAGGGAGGAACGCTGAGAAAGTGGCCCACATGCTCAGGCACTTTGACTTCCTAAAGATCCACGATTTGTTCCTGAGCTTCGATGACACCCCTCTTGGGGCAGCCTCCCTGGCCCAGGTCCACAAGGCGGTGTTGCATGATGGTCGGACAGTGGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-205-5p(1), mmu-miR-106b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGACTTCCTAAAGATCCACGATTTGT
siRNA sets
CAAATCGTGGATCTTTAGG, AAATCGTGGATCTTTAGGA, ACAAATCGTGGATCTTTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADCK1_318_ORF_1
1.2952
0.392800853
0.903968461
483
ORF sequence:
ATGGAGGTGCTTGTCCTGGCCGTGAAGCAACTTTTCCCAGATTTTGAATTCATGTGGCTGGTGGATGAAGCGAAGAAGAACCTGCCTCTCGAGTTGGACTTCCTGAATGAAGGGAGGAACGCTGAGAAAGTGGCCCACATGCTCAGGCACTTTGACTTCCTAAAGATCCACGATTTGTTCCTGAGCTTCGATGACACCCCTCTTGGGGCAGCCTCCCTGGCCCAGGTCCACAAGGCGGTGTTGCATGATGGTCGGACAGTGGCAGTTAAGGTCCAGCACCCGAAGGTTCAGGCTCAAAGCTCTAAGGACATTCTCCTGATGGAGGTGCTTGTCCTGGCCGTGAAGCAACTTTTCCCAGATTTTGAATTCATGTGGCTGGTGGATGAAGCGAAGAAGAACCTGCCTCTCGAGTTGGACTTCCTGAATGAAGGGAGGAACGCTGAGAAAGTGGCCCACATGCTCAGGCACTTTGACTTCCTAAAG
Peptide sequence:
MEVLVLAVKQLFPDFEFMWLVDEAKKNLPLELDFLNEGRNAEKVAHMLRHFDFLKIHDLFLSFDDTPLGAASLAQVHKAVLHDGRTVAVKVQHPKVQAQSSKDILLMEVLVLAVKQLFPDFEFMWLVDEAKKNLPLELDFLNEGRNAEKVAHMLRHFDFLK
IRESfinder result
IRESfinder score
0.350592454
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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