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mmu_circAdam9_567
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam9_567
mm39_Chr. Position ID:
chr8|25463044|25472138|-
Gene name:
Adam9
Transcript:
ENSMUST00000084035.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
15,14,13,12
Exon/intron offsets:
0,5399,6394,8922
Genomic length:
9094
Exon/intron sizes in nt:
10,61,96,93,172
Flanking exon/introns:
chr8:25460785-25463044|chr8:25472138-25480282
Splice length:
567
Other database IDs:
CIRCpedia: MMU_CIRCpedia_14333, circAtlas: mmu-Adam9_0004
CIRCpedia: HSA_CIRCpedia_89128
Human Ortholog IDs:
Mature spliced Sequence:
CGGGTCCAGAAACTTTAGCAGTTGCAGTGCGGAGGACTTTGAGAAGTTAACGTTGAATAAGGGAGGAAGCTGCCTGCTTAACATCCCGAAGCCCGACGAAGCCTACAGCGCGCCCTCCTGTGGTAATAAGCTGGTGGACCCTGGAGAGGAGTGTGACTGCGGCACAGCGAAGGAGTGTGAGGTGGACCCATGCTGTGAAGGAAGCACTTGTAAGCTCAAGTCATTTGCTGAGTGTGCATATGGCGACTGTTGTAAAGATTGCCAGTTCCTTCCAGGAGGCTCCATGTGCAGAGGGAAGACCAGTGAGTGTGATGTTCCTGAGTACTGCAACGGTTCCTCTCAGTTCTGCCCGCCAGATGTCTTCATTCAGAATGGATATCCTTGCCAGAACAGCAAAGCCTACTGCTACAATGGCATGTGCCAATATTATGACGCGCAGTGTCAGGTCATCTTTGGTTCAAAGGCTAAGGCTGCCCCAAGAGATTGCTTCATTGAAGTCAATTCTAAAGGTGACAGATTTGGCAACTGTGGTTTCTCCGGCAGTGAGTACAAGAAGTGTGCCACTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACTGTGGTTTCTCCGGCAG
60.54
GTCGGGCTTCGGGATGTTAA
60.11
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACTGTGGTTTCTCCGGCAG
60.54
TCGGGCTTCGGGATGTTAAG
59.82
140
PCR template sequence:
AGGCTGCCCCAAGAGATTGCTTCATTGAAGTCAATTCTAAAGGTGACAGATTTGGCAACTGTGGTTTCTCCGGCAGTGAGTACAAGAAGTGTGCCACTGGCGGGTCCAGAAACTTTAGCAGTTGCAGTGCGGAGGACTTTGAGAAGTTAACGTTGAATAAGGGAGGAAGCTGCCTGCTTAACATCCCGAAGCCCGACGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-434-3p(1), mmu-miR-96-5p(1), mmu-miR-212-3p(1), mmu-miR-324-3p(1), mmu-miR-666-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTGTGCCACTGGCGGGTCCAGAAAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM9_567_ORF_1
1.1781
0.241960374
0.982658713
849
ORF sequence:
ATGTGCAGAGGGAAGACCAGTGAGTGTGATGTTCCTGAGTACTGCAACGGTTCCTCTCAGTTCTGCCCGCCAGATGTCTTCATTCAGAATGGATATCCTTGCCAGAACAGCAAAGCCTACTGCTACAATGGCATGTGCCAATATTATGACGCGCAGTGTCAGGTCATCTTTGGTTCAAAGGCTAAGGCTGCCCCAAGAGATTGCTTCATTGAAGTCAATTCTAAAGGTGACAGATTTGGCAACTGTGGTTTCTCCGGCAGTGAGTACAAGAAGTGTGCCACTGGCGGGTCCAGAAACTTTAGCAGTTGCAGTGCGGAGGACTTTGAGAAGTTAACGTTGAATAAGGGAGGAAGCTGCCTGCTTAACATCCCGAAGCCCGACGAAGCCTACAGCGCGCCCTCCTGTGGTAATAAGCTGGTGGACCCTGGAGAGGAGTGTGACTGCGGCACAGCGAAGGAGTGTGAGGTGGACCCATGCTGTGAAGGAAGCACTTGTAAGCTCAAGTCATTTGCTGAGTGTGCATATGGCGACTGTTGTAAAGATTGCCAGTTCCTTCCAGGAGGCTCCATGTGCAGAGGGAAGACCAGTGAGTGTGATGTTCCTGAGTACTGCAACGGTTCCTCTCAGTTCTGCCCGCCAGATGTCTTCATTCAGAATGGATATCCTTGCCAGAACAGCAAAGCCTACTGCTACAATGGCATGTGCCAATATTATGACGCGCAGTGTCAGGTCATCTTTGGTTCAAAGGCTAAGGCTGCCCCAAGAGATTGCTTCATTGAAGTCAATTCTAAAGGTGACAGATTTGGCAACTGTGGTTTCTCCGGCAGTGAGTACAAGAAGTGTGCCACT
Peptide sequence:
MCRGKTSECDVPEYCNGSSQFCPPDVFIQNGYPCQNSKAYCYNGMCQYYDAQCQVIFGSKAKAAPRDCFIEVNSKGDRFGNCGFSGSEYKKCATGGSRNFSSCSAEDFEKLTLNKGGSCLLNIPKPDEAYSAPSCGNKLVDPGEECDCGTAKECEVDPCCEGSTCKLKSFAECAYGDCCKDCQFLPGGSMCRGKTSECDVPEYCNGSSQFCPPDVFIQNGYPCQNSKAYCYNGMCQYYDAQCQVIFGSKAKAAPRDCFIEVNSKGDRFGNCGFSGSEYKKCAT
IRESfinder result
IRESfinder score
0.376018049
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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