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mmu_circAdam9_215
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam9_215
mm39_Chr. Position ID:
chr8|25487758|25494853|-
Gene name:
Adam9
Transcript:
ENSMUST00000084035.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,5515,7036
Genomic length:
7095
Exon/intron sizes in nt:
77,79,59
Flanking exon/introns:
chr8:25486866-25487758|chr8:25494853-25496192
Splice length:
215
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATCTCTTACGTCATCCAGGCCCAAGGAAAACAGCATATTATTCACTTGGAAAGAAACACAGACCTTTTACCTAATGATTTTGTAGTTTACACCTACGACAAGGAAGGCTCCCTACTCTCTGACCATCCCAACGTACAGAGCCATTGTCACTATCGAGGCTATGTGGAGGGAGTGCAGAATTCCGCGGTTGCTGTGAGCGCCTGCTTTGGACTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATCCCAACGTACAGAGCCA
59.75
GGTCTGTGTTTCTTTCCAAGTGA
59.05
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCATCCCAACGTACAGAGC
59.75
GGTCTGTGTTTCTTTCCAAGTGA
59.05
158
PCR template sequence:
TCTCTGACCATCCCAACGTACAGAGCCATTGTCACTATCGAGGCTATGTGGAGGGAGTGCAGAATTCCGCGGTTGCTGTGAGCGCCTGCTTTGGACTCAGATCTCTTACGTCATCCAGGCCCAAGGAAAACAGCATATTATTCACTTGGAAAGAAACACAGACCTTTTACCTAATGATTTTGTAGTTTACACCTACGACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-1199-5p(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTTTGGACTCAGATCTCTTACGTCA
siRNA sets
AAGAGATCTGAGTCCAAAG, TGACGTAAGAGATCTGAGT, AGAGATCTGAGTCCAAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.690123909
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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