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mmu_circAdam32_701
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam32_701
mm39_Chr. Position ID:
chr8|25374453|25392900|-
Gene name:
Adam32
Transcript:
ENSMUST00000121438.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
14,13,12,11,10
Exon/intron offsets:
0,1783,14016,17004,18365
Genomic length:
18447
Exon/intron sizes in nt:
199,105,178,137,82
Flanking exon/introns:
chr8:25368845-25374453|chr8:25392900-25403974
Splice length:
701
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTATAACGAGTACCCAAATTACATGGGAGCAACGTATCCTGGGAAGATGTGTACTGCCCACTACTCTGCAGGAATCACAATGTACCCCAAGGATATGACCTTGGAGGCCTTTTCGGTTATTCTCACCCAAATGCTGGGGCTCAGTCTGGGGATATCGTATGATGAGCCTGAGAAATGCTACTGCTCGGAATCCATCTGCATCATGAATCCCAGGGCGATGCAATATGGAGGTGTGAAGTCATTTAGCAATTGCAGTTTGAATGACTTTGAACATTTTAAATCGAACGAGGGTGCCAAATGTCTGCAGAATAAGCCACAGATGCAAAGGACTGCAGCAGCGGTCTGCGGAAATGGCAAGGTGGAGGGTGATGAAATTTGTGACTGTGGCTCTGAAGCAGAATGTGGGCCTGATAGCTGTTGTGAACCTAACAGATGTGTTCTGAAAGCAGGAAGAGCTTGTGACAGTAAGTCTCCCTCATCAACATGCTGCAAAAATTGTCAATTTTTACCAGAAAAACATCAATGTAGGCCGGAAAAGCATCTATACTGTGACATTCCTGAAGTCTGCAATGGGAGCTCAGGAAATTGTCCACCTGATGTAACTATAAACAATGGGCATGTTTGCAAAGAAAGCGGGACTATCTGTTATAATGGAGACTGTCCGGACCTGGACAGGGTGTGCGAGTCAATATATGGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTTTGCAAAGAAAGCGGGACT
59.93
ATTCCTGCAGAGTAGTGGGC
59.46
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTTGCAAAGAAAGCGGGA
58.89
ATTCCTGCAGAGTAGTGGGC
59.46
158
PCR template sequence:
ACTATAAACAATGGGCATGTTTGCAAAGAAAGCGGGACTATCTGTTATAATGGAGACTGTCCGGACCTGGACAGGGTGTGCGAGTCAATATATGGAGCAGTTATAACGAGTACCCAAATTACATGGGAGCAACGTATCCTGGGAAGATGTGTACTGCCCACTACTCTGCAGGAATCACAATGTACCCCAAGGATATGACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-1a-3p(1), mmu-miR-339-5p(1), mmu-miR-10a-5p(1), mmu-miR-541-5p(1), mmu-miR-362-5p(1), mmu-miR-342-5p(1), mmu-miR-129-5p(1), mmu-miR-452-3p(1), mmu-miR-350-3p(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATATATGGAGCAGTTATAACGAGTAC
siRNA sets
TACTCGTTATAACTGCTCC, GTACTCGTTATAACTGCTC, ACTCGTTATAACTGCTCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM32_701_ORF_1
0.6084
-0.00090189
0.811364272
777
ORF sequence:
ATGGGAGCAACGTATCCTGGGAAGATGTGTACTGCCCACTACTCTGCAGGAATCACAATGTACCCCAAGGATATGACCTTGGAGGCCTTTTCGGTTATTCTCACCCAAATGCTGGGGCTCAGTCTGGGGATATCGTATGATGAGCCTGAGAAATGCTACTGCTCGGAATCCATCTGCATCATGAATCCCAGGGCGATGCAATATGGAGGTGTGAAGTCATTTAGCAATTGCAGTTTGAATGACTTTGAACATTTTAAATCGAACGAGGGTGCCAAATGTCTGCAGAATAAGCCACAGATGCAAAGGACTGCAGCAGCGGTCTGCGGAAATGGCAAGGTGGAGGGTGATGAAATTTGTGACTGTGGCTCTGAAGCAGAATGTGGGCCTGATAGCTGTTGTGAACCTAACAGATGTGTTCTGAAAGCAGGAAGAGCTTGTGACAGTAAGTCTCCCTCATCAACATGCTGCAAAAATTGTCAATTTTTACCAGAAAAACATCAATGTAGGCCGGAAAAGCATCTATACTGTGACATTCCTGAAGTCTGCAATGGGAGCTCAGGAAATTGTCCACCTGATGTAACTATAAACAATGGGCATGTTTGCAAAGAAAGCGGGACTATCTGTTATAATGGAGACTGTCCGGACCTGGACAGGGTGTGCGAGTCAATATATGGAGCAGTTATAACGAGTACCCAAATTACATGGGAGCAACGTATCCTGGGAAGATGTGTACTGCCCACTACTCTGCAGGAATCACAATGTACCCCAAGGATATGA
Peptide sequence:
MGATYPGKMCTAHYSAGITMYPKDMTLEAFSVILTQMLGLSLGISYDEPEKCYCSESICIMNPRAMQYGGVKSFSNCSLNDFEHFKSNEGAKCLQNKPQMQRTAAAVCGNGKVEGDEICDCGSEAECGPDSCCEPNRCVLKAGRACDSKSPSSTCCKNCQFLPEKHQCRPEKHLYCDIPEVCNGSSGNCPPDVTINNGHVCKESGTICYNGDCPDLDRVCESIYGAVITSTQITWEQRILGRCVLPTTLQESQCTPRI
IRESfinder result
IRESfinder score
0.380326452
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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