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mmu_circAdam32_521
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam32_521
mm39_Chr. Position ID:
chr8|25406303|25428185|-
Gene name:
Adam32
Transcript:
ENSMUST00000121438.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,3622,5000,5972,7777,21817
Genomic length:
21882
Exon/intron sizes in nt:
69,172,77,76,62,65
Flanking exon/introns:
chr8:25404406-25406303|chr8:25428185-25438735
Splice length:
521
Other database IDs:
CIRCpedia: MMU_CIRCpedia_219002, circAtlas: mmu-Adam32_0020
circBase: hsa_circ_0136517, circAtlas: hsa-ADAM32_0016, CIRCpedia: HSA_CIRCpedia_141375
Human Ortholog IDs:
Mature spliced Sequence:
AGTCTCAAAGTTCATTTCTAGAAATCATATTTCCAGAGAAAATTGAAGATAAAACACACTCAGAAGAACAGATATCCTATATTATTCCAATAAACAAGAAGCAGTACACTGTGCACCTCCAGAAAAGATATTTTTTAACGAATCGTTTTATGGTTTATATGTATAATCAAGGATCTACAAGTTTTCATTCTCCAAACATTCCGGCTCAGTGCTATTATCAAGGACACATCAAAGGCTACCCGAACTCAGTCGCCACCCTCAGCACATGCTCTGGACTGAGAGGGTTCCTGCAGTTTGAGAATGTGTCCTACGGAATTGAGCCTCTGCAATCTGCATTTACATCTCAACACATTGTTTATAAGCTAGGGAACAAAGAGAAGGAGTTGATATTTAACAAAAACAGCAGAAACATAGAGATGCCTACAAACTATGGCATTTTAATCAACAAAAAGCCAAAATCACCTTTTAAAAACTTGTTTCCCCTCTATCTAGAGATGAGTATTGTGGTGGACAAAGCGCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAACAAAAAGCCAAAATCACCT
57.9
AGGATATCTGTTCTTCTGAGTGTGT
59.28
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAACAAAAAGCCAAAATCACCT
57.9
TCTGTTCTTCTGAGTGTGTTT
55.03
152
PCR template sequence:
TACAAACTATGGCATTTTAATCAACAAAAAGCCAAAATCACCTTTTAAAAACTTGTTTCCCCTCTATCTAGAGATGAGTATTGTGGTGGACAAAGCGCTGAGTCTCAAAGTTCATTTCTAGAAATCATATTTCCAGAGAAAATTGAAGATAAAACACACTCAGAAGAACAGATATCCTATATTATTCCAATAAACAAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-206-3p(1), mmu-miR-128-3p(1), mmu-miR-24-3p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACAAAGCGCTGAGTCTCAAAGTTC
siRNA sets
GAACTTTGAGACTCAGCGC, AACTTTGAGACTCAGCGCT, TTTGAGACTCAGCGCTTTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM32_521_ORF_1
0.7236
-0.017641187
0.239038548
393
ORF sequence:
ATGGTTTATATGTATAATCAAGGATCTACAAGTTTTCATTCTCCAAACATTCCGGCTCAGTGCTATTATCAAGGACACATCAAAGGCTACCCGAACTCAGTCGCCACCCTCAGCACATGCTCTGGACTGAGAGGGTTCCTGCAGTTTGAGAATGTGTCCTACGGAATTGAGCCTCTGCAATCTGCATTTACATCTCAACACATTGTTTATAAGCTAGGGAACAAAGAGAAGGAGTTGATATTTAACAAAAACAGCAGAAACATAGAGATGCCTACAAACTATGGCATTTTAATCAACAAAAAGCCAAAATCACCTTTTAAAAACTTGTTTCCCCTCTATCTAGAGATGAGTATTGTGGTGGACAAAGCGCTGAGTCTCAAAGTTCATTTCTAG
Peptide sequence:
MVYMYNQGSTSFHSPNIPAQCYYQGHIKGYPNSVATLSTCSGLRGFLQFENVSYGIEPLQSAFTSQHIVYKLGNKEKELIFNKNSRNIEMPTNYGILINKKPKSPFKNLFPLYLEMSIVVDKALSLKVHF
IRESfinder result
IRESfinder score
0.754847859
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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