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mmu_circAdam22_939
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_939
mm39_Chr. Position ID:
chr5|8180484|8199282|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
19,18,17,16,15,14,13,12,11,10,9
Exon/intron offsets:
0,1914,4027,4328,6321,11234,12557,13736,15068,15970,18723
Genomic length:
18798
Exon/intron sizes in nt:
51,81,93,72,100,52,91,85,167,72,75
Flanking exon/introns:
chr5:8180272-8180484|chr5:8199282-8202378
Splice length:
939
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTTCTTCGATTTCCTCGGAATGTAGAGGAGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAATTCGAGCCAGTGTGCC
59.83
GCCGGTGCTTTTTAAACATGAG
59.01
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAATTCGAGCCAGTGTGC
59.83
GCCGGTGCTTTTTAAACATGAG
59.01
157
PCR template sequence:
AGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGCTTCTTCGATTTCCTCGGAATGTAGAGGAGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGTTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(2), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-let-7b-5p(1), mmu-miR-18a-5p(1), mmu-miR-503-5p(1), mmu-miR-433-3p(1), mmu-miR-382-3p(1), mmu-miR-501-3p(1), mmu-miR-207(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGATGGTACTCAGCTTCTTCGATTTC
siRNA sets
AATCGAAGAAGCTGAGTAC, GAAATCGAAGAAGCTGAGT, ATCGAAGAAGCTGAGTACC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_939_ORF_1
0.9697
0.160599168
0.999968236
1827
ORF sequence:
ATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGCTTCTTCGATTTCCTCGGAATGTAGAGGAGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAG
Peptide sequence:
MIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLKTRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLLRGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCKKCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQLLRFPRNVEEETKYIELMIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLKTRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLLRGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCKKCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQ
IRESfinder result
IRESfinder score
0.541011686
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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