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mmu_circAdam22_797
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_797
mm39_Chr. Position ID:
chr5|8161894|8182479|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
25,24,23,22,21,20,19,18
Exon/intron offsets:
0,4650,5694,9153,15432,18314,18590,20504
Genomic length:
20585
Exon/intron sizes in nt:
91,115,169,120,106,64,51,81
Flanking exon/introns:
chr5:8157019-8161894|chr5:8182479-8184511
Splice length:
797
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAATAATTGCCGGCACCA
60.03
GCACACTGGCTCGAATTTCC
59.83
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAATAATTGCCGGCACCA
60.03
GGCACACTGGCTCGAATTTC
59.83
153
PCR template sequence:
CTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAATTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-7b-5p(1), mmu-miR-125b-5p(1), mmu-miR-339-5p(1), mmu-miR-363-3p(1), mmu-miR-125a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-207(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGGGGTTATAATTTCAGCCTCTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_797_ORF_1
0.9361
0.092852334
0.893064036
732
ORF sequence:
ATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAATTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGA
Peptide sequence:
MDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKVTASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRTLNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVCNRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYNFSLWGPCAERQ
IRESfinder result
IRESfinder score
0.537252888
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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