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mmu_circAdam22_467
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_467
mm39_Chr. Position ID:
chr5|8191718|8196526|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
14,13,12,11,10
Exon/intron offsets:
0,1323,2502,3834,4736
Genomic length:
4808
Exon/intron sizes in nt:
52,91,85,167,72
Flanking exon/introns:
chr5:8186905-8191718|chr5:8196526-8199207
Splice length:
467
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
TTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCCACAATGTCGGTATAA
56.61
TGTTCACCACAGACTTCGCA
59.82
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCCACAATGTCGGTATAATCT
59.61
TGTTCACCACAGACTTCGCA
59.82
160
PCR template sequence:
TAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(1), mmu-miR-503-5p(1), mmu-miR-501-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
TGGGAGACACCGGTTTAAAAAGCACC
siRNA sets
TTTTTAAACCGGTGTCTCC, TTTTAAACCGGTGTCTCCC, CTTTTTAAACCGGTGTCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_467_ORF_1
0.7978
0.042652467
0.437489948
465
ORF sequence:
ATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGA
Peptide sequence:
MADVIYKDQLKTRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLLRGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGLKSTGFLLYILIPMRSLW
IRESfinder result
IRESfinder score
0.527525681
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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