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mmu_circAdam22_289
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_289
mm39_Chr. Position ID:
chr5|8167588|8171167|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
23,22
Exon/intron offsets:
0,3459
Genomic length:
3579
Exon/intron sizes in nt:
1,69,120
Flanking exon/introns:
chr5:8166659-8167588|chr5:8171167-8177326
Splice length:
289
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTTGGAACACAGGTGTCT
59.45
GCCTTGGGATGTTGCCAATG
60.11
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGCTTGGAACACAGGTGTC
59.34
GCCTTGGGATGTTGCCAATG
60.11
144
PCR template sequence:
TCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCAGGAAATGGAGGATGTACTTTGT
siRNA sets
AAGTACATCCTCCATTTCC, AAAGTACATCCTCCATTTC, GTACATCCTCCATTTCCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_289_ORF_1
0.8095
0.129366401
0.2496012
294
ORF sequence:
ATGGAGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGA
Peptide sequence:
MEDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRTLNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNG
IRESfinder result
IRESfinder score
0.654023512
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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