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mmu_circAdam22_284
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_284
mm39_Chr. Position ID:
chr5|8208826|8282676|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,6,5,4
Exon/intron offsets:
0,1909,8500,73783
Genomic length:
73850
Exon/intron sizes in nt:
70,64,83,67
Flanking exon/introns:
chr5:8202449-8208826|chr5:8282676-8379979
Splice length:
284
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_59192
Human Ortholog IDs:
Mature spliced Sequence:
CGAGTTGCTGTCGTCAGGTTATGTAGAGAGACAGATTGAACATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATTCGAGGAAACCCTGTCTCATTTGTTGCCCTATCAACATGCCATGGACTCCATGGGATGTTCTATGATGGAAACCACACATATCTCATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAAATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCGTCTCATTGTCACTCCGT
60.07
TCCTCCTTTGTTCTCCACGAC
59.66
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGTCTCATTGTCACTCCGTG
60.07
TCCTCCTTTGTTCTCCACGAC
59.66
140
PCR template sequence:
ATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAAATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTGCGAGTTGCTGTCGTCAGGTTATGTAGAGAGACAGATTGAACATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-29b-2-5p(1), mmu-miR-466a-5p(1), mmu-miR-466p-5p(1), mmu-miR-190b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACCTTCCGTCTGCGAGTTGCTGTCG
siRNA sets
AACTCGCAGACGGAAGGTC, CAACTCGCAGACGGAAGGT, GACAGCAACTCGCAGACGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_284_ORF_1
0.7845
-0.007039214
0.142914431
282
ORF sequence:
ATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATTCGAGGAAACCCTGTCTCATTTGTTGCCCTATCAACATGCCATGGACTCCATGGGATGTTCTATGATGGAAACCACACATATCTCATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAAATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTGCGAGTTGCTGTCGTCAGGTTATGTAGAGAGACAGATTGA
Peptide sequence:
MEARSWRTKEENTVITRARFEETLSHLLPYQHAMDSMGCSMMETTHISLSQKKMRSLKSRLIVTPCTNPDSLNFPWMTFRLRVAVVRLCRETD
IRESfinder result
IRESfinder score
0.572931649
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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