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mmu_circAdam22_1861
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_1861
mm39_Chr. Position ID:
chr5|8142339|8202449|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
21
Exon/intron indexes:
28,27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8
Exon/intron offsets:
0,391,14662,19555,24205,25249,28708,34987,37869,38145,40059,42172,42473,44466,49379,50702,51881,53213,54115,56868,60039
Genomic length:
60110
Exon/intron sizes in nt:
67,101,18,91,115,169,120,106,64,51,81,93,72,100,52,91,85,167,72,75,71
Flanking exon/introns:
chr5:8132269-8142339|chr5:8202449-8208826
Splice length:
1861
Other database IDs:
circAtlas: mmu-Adam22_0012
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_121670
Human Ortholog IDs:
Mature spliced Sequence:
AATTTCAACGCGTAAACATTACTCCCCCTCAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGGAGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGACCTCGCAGTAACTCC
59.54
GGCTTCAAAATAAACTGAGGGGG
59.8
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGTAACTCCTGGCAAGGT
59.96
GAGGAAATCGAAGAAGCTGTCT
58.14
172
PCR template sequence:
AAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGAAATTTCAACGCGTAAACATTACTCCCCCTCAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(2), mmu-miR-207(2), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-7b-5p(1), mmu-let-7b-5p(1), mmu-miR-18a-5p(1), mmu-miR-125b-5p(1), mmu-miR-339-5p(1), mmu-miR-503-5p(1), mmu-miR-433-3p(1), mmu-miR-363-3p(1), mmu-miR-125a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-382-3p(1), mmu-miR-501-3p(1), mmu-miR-5099(1), mmu-miR-3065-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTAACTCAACTGAAATTTCAACGCGT
siRNA sets
GTTGAAATTTCAGTTGAGT, GAAATTTCAGTTGAGTTAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_1861_ORF_1
0.817
0.106926912
0.999986568
2022
ORF sequence:
ATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGAAATTTCAACGCGTAAACATTACTCCCCCTCAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGGAGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGTTGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAAACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCACTGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCTTTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTGAGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCCACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTCCGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACT
Peptide sequence:
MDSLILGVKGFQTQNIFQTSVKMGDLAVTPGKVTWEATKRKSEGKDLDLDLTQLKFQRVNITPPQFILKPRLKRRKRQLLRFPRNVEEETKYIELMIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLKTRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLLRGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCKKCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKVTASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRTLNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVCNRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNMGGNKKKIRGKRFRPRSNST
IRESfinder result
IRESfinder score
0.568539798
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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