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mmu_circAcvr2a_1292
Basic information of circular RNA
PanCircBase ID:
mmu_circAcvr2a_1292
mm39_Chr. Position ID:
chr2|48760300|48788620|+
Gene name:
Acvr2a
Transcript:
ENSMUST00000063886.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
2,3,4,5,6,7,8,9,10
Exon/intron offsets:
0,2768,3030,20009,21840,23218,24377,26683,28189
Genomic length:
28320
Exon/intron sizes in nt:
20,81,10,15,51,44,14,40,00,00,00,00,000
Flanking exon/introns:
chr2:48704838-48760300|chr2:48788620-48789605
Splice length:
1292
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGCTATACTTGGCAGATCAGAAACTCAGGAGTGTCTTTTCTTTAATGCTAATTGGGAAAGAGACAGAACCAACCAGACTGGTGTTGAACCTTGCTATGGTGATAAAGATAAACGGCGACATTGTTTTGCTACCTGGAAGAATATTTCTGGTTCCATTGAAATAGTGAAGCAAGGTTGTTGGCTGGATGATATCAACTGCTATGACAGGACTGATTGTATAGAAAAAAAAGACAGCCCTGAAGTGTACTTTTGTTGCTGTGAGGGCAATATGTGTAATGAAAAGTTCTCTTATTTTCCGGAGATGGAAGTCACACAGCCCACTTCAAATCCTGTTACACCGAAGCCACCCTATTACAACATTCTGCTGTATTCCTTGGTACCACTAATGTTAATTGCAGGAATTGTCATTTGTGCATTTTGGGTGTACAGACATCACAAGATGGCCTACCCTCCTGTACTTGTTCCTACTCAAGACCCAGGACCACCCCCACCTTCCCCATTACTAGGGTTGAAGCCATTGCAGCTGTTAGAAGTGAAAGCAAGGGGAAGATTTGGTTGTGTCTGGAAAGCCCAGTTGCTCAATGAATATGTGGCTGTCAAAATATTTCCAATACAGGACAAACAGTCCTGGCAGAATGAATATGAAGTCTATAGTCTACCTGGAATGAAGCATGAGAACATACTACAGTTCATTGGTGCAGAGAAAAGAGGCACCAGTGTGGATGTGGACCTGTGGCTAATCACAGCATTTCATGAAAAGGGCTCACTGTCAGACTTTCTTAAGGCTAATGTGGTCTCTTGGAATGAACTTTGTCATATTGCAGAAACCATGGCTAGAGGATTGGCATATTTACATGAGGATATACCTGGCTTAAAAGATGGCCACAAGCCTGCAATCTCTCACAGGGACATCAAAAGTAAAAATGTGCTGTTGAAAAACAATCTGACAGCTTGCATTGCTGACTTTGGGTTGGCCTTAAAGTTCGAGGCTGGCAAGTCTGCAGGTGACACCCATGGGCAGGTTGGTACCCGGAGGTATATGGCTCCAGAGGTGTTGGAGGGTGCTATAAACTTCCAAAGGGACGCATTTCTGAGGATAGATATGTACGCCATGGGATTAGTCCTATGGGAATTGGCTTCTCGTTGCACTGCTGCAGATGGACCCGTAGATGAGTACATGTTACCATTTGAGGAAGAAATTGGCCAGCATCCATCTCTTGAAGATATGCAGGAAGTTGTTGTGCATAAAAAAAAGAGGCCTGTTTTAAGAGATTATTGGCAGAAACATGCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCCAGCATCCATCTCTTG
60.11
CCAGTCTGGTTGGTTCTGTCT
59.58
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCAGCATCCATCTCTTGA
59.82
CCAGTCTGGTTGGTTCTGTCT
59.58
172
PCR template sequence:
GGAAGAAATTGGCCAGCATCCATCTCTTGAAGATATGCAGGAAGTTGTTGTGCATAAAAAAAAGAGGCCTGTTTTAAGAGATTATTGGCAGAAACATGCAGTGCTATACTTGGCAGATCAGAAACTCAGGAGTGTCTTTTCTTTAATGCTAATTGGGAAAGAGACAGAACCAACCAGACTGGTGTTGAACCTTGCTATGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(2), mmu-miR-135a-5p(1), mmu-miR-204-5p(1), mmu-miR-130a-3p(1), mmu-miR-148b-3p(1), mmu-miR-433-5p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-721(1), mmu-miR-301a-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-802-5p(1), mmu-miR-207(1), mmu-miR-3065-5p(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAGAAACATGCAGTGCTATACTTGG
siRNA sets
TATAGCACTGCATGTTTCT, AAGTATAGCACTGCATGTT, CAAGTATAGCACTGCATGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACVR2A_1292_ORF_1
0.8815
0.020533671
0.993258623
1188
ORF sequence:
ATGTGTAATGAAAAGTTCTCTTATTTTCCGGAGATGGAAGTCACACAGCCCACTTCAAATCCTGTTACACCGAAGCCACCCTATTACAACATTCTGCTGTATTCCTTGGTACCACTAATGTTAATTGCAGGAATTGTCATTTGTGCATTTTGGGTGTACAGACATCACAAGATGGCCTACCCTCCTGTACTTGTTCCTACTCAAGACCCAGGACCACCCCCACCTTCCCCATTACTAGGGTTGAAGCCATTGCAGCTGTTAGAAGTGAAAGCAAGGGGAAGATTTGGTTGTGTCTGGAAAGCCCAGTTGCTCAATGAATATGTGGCTGTCAAAATATTTCCAATACAGGACAAACAGTCCTGGCAGAATGAATATGAAGTCTATAGTCTACCTGGAATGAAGCATGAGAACATACTACAGTTCATTGGTGCAGAGAAAAGAGGCACCAGTGTGGATGTGGACCTGTGGCTAATCACAGCATTTCATGAAAAGGGCTCACTGTCAGACTTTCTTAAGGCTAATGTGGTCTCTTGGAATGAACTTTGTCATATTGCAGAAACCATGGCTAGAGGATTGGCATATTTACATGAGGATATACCTGGCTTAAAAGATGGCCACAAGCCTGCAATCTCTCACAGGGACATCAAAAGTAAAAATGTGCTGTTGAAAAACAATCTGACAGCTTGCATTGCTGACTTTGGGTTGGCCTTAAAGTTCGAGGCTGGCAAGTCTGCAGGTGACACCCATGGGCAGGTTGGTACCCGGAGGTATATGGCTCCAGAGGTGTTGGAGGGTGCTATAAACTTCCAAAGGGACGCATTTCTGAGGATAGATATGTACGCCATGGGATTAGTCCTATGGGAATTGGCTTCTCGTTGCACTGCTGCAGATGGACCCGTAGATGAGTACATGTTACCATTTGAGGAAGAAATTGGCCAGCATCCATCTCTTGAAGATATGCAGGAAGTTGTTGTGCATAAAAAAAAGAGGCCTGTTTTAAGAGATTATTGGCAGAAACATGCAGTGCTATACTTGGCAGATCAGAAACTCAGGAGTGTCTTTTCTTTAATGCTAATTGGGAAAGAGACAGAACCAACCAGACTGGTGTTGAACCTTGCTATGGTGATAAAGATAAACGGCGACATTGTTTTGCTACCTGGAAGAATATTTCTGGTTCCATTGAAATAG
Peptide sequence:
MCNEKFSYFPEMEVTQPTSNPVTPKPPYYNILLYSLVPLMLIAGIVICAFWVYRHHKMAYPPVLVPTQDPGPPPPSPLLGLKPLQLLEVKARGRFGCVWKAQLLNEYVAVKIFPIQDKQSWQNEYEVYSLPGMKHENILQFIGAEKRGTSVDVDLWLITAFHEKGSLSDFLKANVVSWNELCHIAETMARGLAYLHEDIPGLKDGHKPAISHRDIKSKNVLLKNNLTACIADFGLALKFEAGKSAGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAMGLVLWELASRCTAADGPVDEYMLPFEEEIGQHPSLEDMQEVVVHKKKRPVLRDYWQKHAVLYLADQKLRSVFSLMLIGKETEPTRLVLNLAMVIKINGDIVLLPGRIFLVPLK
IRESfinder result
IRESfinder score
0.601155255
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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