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mmu_circAcvr1_1516
Basic information of circular RNA
PanCircBase ID:
mmu_circAcvr1_1516
mm39_Chr. Position ID:
chr2|58348896|58397533|-
Gene name:
Acvr1
Transcript:
ENSMUST00000090935.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
11,10,9,8,7,6,5,4
Exon/intron offsets:
0,3996,9629,15288,18720,20780,41599,48392
Genomic length:
48637
Exon/intron sizes in nt:
19,82,76,14,71,00,21,20,00,00,000
Flanking exon/introns:
chr2:58338456-58348896|chr2:58397533-58406035
Splice length:
1516
Other database IDs:
circAtlas: mmu-Acvr1_0022
Human Ortholog IDs:
Mature spliced Sequence:
GATGAATGGATCCAGGGTCTGGTTTTAAGTTCTATGGTAGTCGTCCAAGGAGCCATTGGTATTCATCTAACGCAAACGATCAAGTTACATTCTGAAAGTAACATCCCACCAGAAACCCTCCAGCAGCAGTCACGTCTGTGTAAAGCCAAGCCCTGGCATGCGCACTGCCAGGTCAGAGTGTGGTGGTACACGTGTTTAACAGGTCATTTGTCAACTGAAGGAAAGACCCCGGCTTGACTTACCTGTTATACAATGGTCGATGGAGTAATGATCCTTCCTGTGCTAATGATGATGGCTTTCCCTTCCCCGAGTGTGGAAGATGAGAAGCCCAAGGTCAACCAGAAACTTTACATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCACTGTGAAGGCCAGCAGTGTTTTTCTTCTCTGAGCATCAACGATGGCTTCCACGTCTACCAGAAGGGCTGCTTTCAGGTTTATGAGCAGGGGAAGATGACGTGTAAGACCCCGCCGTCACCTGGCCAGGCTGTGGAGTGCTGCCAAGGGGACTGGTGTAACAGGAACATCACGGCCCAGCTGCCCACTAAAGGGAAGTCCTTCCCCGGAACACAGAATTTCCACCTGGAAGTTGGCCTTATCATCCTCTCGGTGGTGTTTGCAGTATGTCTTTTAGCTTGCATCCTTGGAGTTGCTCTCAGGAAGTTTAAGAGACGCAATCAAGAGCGCCTGAACCCCAGAGACGTGGAGTATGGTACCATTGAAGGGCTCATCACCACCAATGTGGGAGACAGCACTCTAGCGGAACTACTAGATCACTCGTGTACATCAGGAAGTGGCTCCGGTCTTCCTTTCCTGGTACAGAGAACGGTGGCTCGCCAGATAACCCTGTTGGAGTGTGTCGGGAAGGGCCGGTATGGAGAAGTATGGAGGGGCAGCTGGCAAGGCGAAAATGTCGCTGTGAAGATCTTCTCCTCCCGAGACGAGAAGTCATGGTTCAGGGAGACGGAATTGTACAACACTGTGATGTTGAGGCATGAAAATATCTTAGGTTTCATCGCTTCAGACATGACCTCCAGACACTCCAGTACCCAGCTGTGGCTCATCACACATTACCATGAAATGGGATCGTTGTATGACTACCTTCAGCTCACTACTCTGGATACGGTTAGCTGCCTTCGGATTGTACTGTCCATAGCCAGCGGCCTTGCCCATTTGCACATAGAGATATTTGGGACCCAAGGGAAGTCCGCCATTGCCCATCGAGATCTGAAGAGCAAAAACATCCTGGTGAAGAAGAATGGACAGTGCTGCATAGCAGATTTGGGCCTGGCAGTCATGCATTCCCAGAGCACAAACCAGCTTGATGTGGGAAACAACCCCCGTGTGGGGACCAAGCGCTACATGGCTCCGGAAGTGCTCGATGAAACCATCCAAGTGGATTGCTTTGATTCTTATAAGAGGGTCGATATTTGGGCCTTTGGCCTTGTTCTGTGGGAAGTGGCCAGGCGAATGGTGAGCAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGGGTCGATATTTGGGCC
59.23
TGCGTTAGATGAATACCAATGGC
59.44
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAAGTGGATTGCTTTGATTCT
58.59
CAATGGCTCCTTGGACGACT
60.04
151
PCR template sequence:
GAAACCATCCAAGTGGATTGCTTTGATTCTTATAAGAGGGTCGATATTTGGGCCTTTGGCCTTGTTCTGTGGGAAGTGGCCAGGCGAATGGTGAGCAATGGATGAATGGATCCAGGGTCTGGTTTTAAGTTCTATGGTAGTCGTCCAAGGAGCCATTGGTATTCATCTAACGCAAACGATCAAGTTACATTCTGAAAGTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(2), mmu-miR-27a-3p(2), mmu-miR-188-3p(2), mmu-miR-134-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-34a-5p(1), mmu-miR-30a-5p(1), mmu-miR-7a-5p(1), mmu-miR-30d-5p(1), mmu-miR-434-3p(1), mmu-miR-411-3p(1), mmu-miR-107-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-342-3p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-3470b(1), mmu-miR-3105-3p(1), mmu-miR-323-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGGTGAGCAATGGATGAATGGATCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACVR1_1516_ORF_1
1.0248
0.257970569
0.99889195
1269
ORF sequence:
ATGGTCGATGGAGTAATGATCCTTCCTGTGCTAATGATGATGGCTTTCCCTTCCCCGAGTGTGGAAGATGAGAAGCCCAAGGTCAACCAGAAACTTTACATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCACTGTGAAGGCCAGCAGTGTTTTTCTTCTCTGAGCATCAACGATGGCTTCCACGTCTACCAGAAGGGCTGCTTTCAGGTTTATGAGCAGGGGAAGATGACGTGTAAGACCCCGCCGTCACCTGGCCAGGCTGTGGAGTGCTGCCAAGGGGACTGGTGTAACAGGAACATCACGGCCCAGCTGCCCACTAAAGGGAAGTCCTTCCCCGGAACACAGAATTTCCACCTGGAAGTTGGCCTTATCATCCTCTCGGTGGTGTTTGCAGTATGTCTTTTAGCTTGCATCCTTGGAGTTGCTCTCAGGAAGTTTAAGAGACGCAATCAAGAGCGCCTGAACCCCAGAGACGTGGAGTATGGTACCATTGAAGGGCTCATCACCACCAATGTGGGAGACAGCACTCTAGCGGAACTACTAGATCACTCGTGTACATCAGGAAGTGGCTCCGGTCTTCCTTTCCTGGTACAGAGAACGGTGGCTCGCCAGATAACCCTGTTGGAGTGTGTCGGGAAGGGCCGGTATGGAGAAGTATGGAGGGGCAGCTGGCAAGGCGAAAATGTCGCTGTGAAGATCTTCTCCTCCCGAGACGAGAAGTCATGGTTCAGGGAGACGGAATTGTACAACACTGTGATGTTGAGGCATGAAAATATCTTAGGTTTCATCGCTTCAGACATGACCTCCAGACACTCCAGTACCCAGCTGTGGCTCATCACACATTACCATGAAATGGGATCGTTGTATGACTACCTTCAGCTCACTACTCTGGATACGGTTAGCTGCCTTCGGATTGTACTGTCCATAGCCAGCGGCCTTGCCCATTTGCACATAGAGATATTTGGGACCCAAGGGAAGTCCGCCATTGCCCATCGAGATCTGAAGAGCAAAAACATCCTGGTGAAGAAGAATGGACAGTGCTGCATAGCAGATTTGGGCCTGGCAGTCATGCATTCCCAGAGCACAAACCAGCTTGATGTGGGAAACAACCCCCGTGTGGGGACCAAGCGCTACATGGCTCCGGAAGTGCTCGATGAAACCATCCAAGTGGATTGCTTTGATTCTTATAAGAGGGTCGATATTTGGGCCTTTGGCCTTGTTCTGTGGGAAGTGGCCAGGCGAATGGTGAGCAATGGATGA
Peptide sequence:
MVDGVMILPVLMMMAFPSPSVEDEKPKVNQKLYMCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGCFQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNFHLEVGLIILSVVFAVCLLACILGVALRKFKRRNQERLNPRDVEYGTIEGLITTNVGDSTLAELLDHSCTSGSGSGLPFLVQRTVARQITLLECVGKGRYGEVWRGSWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGFIASDMTSRHSSTQLWLITHYHEMGSLYDYLQLTTLDTVSCLRIVLSIASGLAHLHIEIFGTQGKSAIAHRDLKSKNILVKKNGQCCIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSYKRVDIWAFGLVLWEVARRMVSNG
IRESfinder result
IRESfinder score
0.441096323
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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