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mmu_circActr1a_202
Basic information of circular RNA
PanCircBase ID:
mmu_circActr1a_202
mm39_Chr. Position ID:
chr19|46372658|46373527|-
Gene name:
Actr1a
Transcript:
ENSMUST00000040270.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,3
Exon/intron offsets:
0,793
Genomic length:
869
Exon/intron sizes in nt:
126,76
Flanking exon/introns:
chr19:46370857-46372658|chr19:46373527-46375890
Splice length:
202
Other database IDs:
circBase: mmu_circ_0000959, CIRCpedia: MMU_CIRCpedia_20857, circAtlas: mmu-Actr1a_0002
circBase: hsa_circ_0019752, circAtlas: hsa-ACTR1A_0002, CIRCpedia: HSA_CIRCpedia_244
Human Ortholog IDs:
Mature spliced Sequence:
TGTGGGCAGACCCAAACATGTTCGTGTCATGGCAGGAGCCCTGGAAGGTGACATCTTCATTGGCCCCAAAGCGGAGGAGCACCGGGGGCTGCTGTCAATCCGTTACCCCATGGAACATGGCATCGTCAAGGACTGGAACGACATGGAACGCATCTGGCAATACGTCTACTCTAAGGACCAGCTGCAGACTTTCTCAGAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGGCATCGTCAAGGACTGG
60.11
GATGTCACCTTCCAGGGCTC
60.11
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCATCGTCAAGGACTGGAA
60.04
AAGATGTCACCTTCCAGGGC
59.67
141
PCR template sequence:
TTACCCCATGGAACATGGCATCGTCAAGGACTGGAACGACATGGAACGCATCTGGCAATACGTCTACTCTAAGGACCAGCTGCAGACTTTCTCAGAGGAGTGTGGGCAGACCCAAACATGTTCGTGTCATGGCAGGAGCCCTGGAAGGTGACATCTTCATTGGCCCCAAAGCGGAGGAGCACCGGGGGCTGCTGTCAATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-425-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTCTCAGAGGAGTGTGGGCAGACCC
siRNA sets
TCTGCCCACACTCCTCTGA, CTGCCCACACTCCTCTGAG, GTCTGCCCACACTCCTCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACTR1A_202_ORF_1
0.8035
0.27115299
0.535684554
387
ORF sequence:
ATGTTCGTGTCATGGCAGGAGCCCTGGAAGGTGACATCTTCATTGGCCCCAAAGCGGAGGAGCACCGGGGGCTGCTGTCAATCCGTTACCCCATGGAACATGGCATCGTCAAGGACTGGAACGACATGGAACGCATCTGGCAATACGTCTACTCTAAGGACCAGCTGCAGACTTTCTCAGAGGAGTGTGGGCAGACCCAAACATGTTCGTGTCATGGCAGGAGCCCTGGAAGGTGACATCTTCATTGGCCCCAAAGCGGAGGAGCACCGGGGGCTGCTGTCAATCCGTTACCCCATGGAACATGGCATCGTCAAGGACTGGAACGACATGGAACGCATCTGGCAATACGTCTACTCTAAGGACCAGCTGCAGACTTTCTCAGAGGAG
Peptide sequence:
MFVSWQEPWKVTSSLAPKRRSTGGCCQSVTPWNMASSRTGTTWNASGNTSTLRTSCRLSQRSVGRPKHVRVMAGALEGDIFIGPKAEEHRGLLSIRYPMEHGIVKDWNDMERIWQYVYSKDQLQTFSEE
IRESfinder result
IRESfinder score
0.265139389
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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