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mmu_circAcsl5_1247
Basic information of circular RNA
PanCircBase ID:
mmu_circAcsl5_1247
mm39_Chr. Position ID:
chr19|55257075|55276394|+
Gene name:
Acsl5
Transcript:
ENSMUST00000043150.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11,12,13
Exon/intron offsets:
0,4125,9263,9437,10820,11797,12692,12806,13829,14664,15759,19184
Genomic length:
19319
Exon/intron sizes in nt:
18,51,09,65,10,21,00,10,00,00,00,00,00,00,000
Flanking exon/introns:
chr19:55242598-55257075|chr19:55276394-55277236
Splice length:
1247
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCTGAATTTCCTGTTGGTATTCACAAAGATGCTTTTTATTTTTAACTTCTTGTTTTCCCCACTTCCAACTCCGGCCCTGATCTGCCTCCTGACGTTTGGAACGGCCATCTTCCTGTGGCTGATCAACAGACCTCAGCCAGTTTTACCTCTCATTGACTTGGACAACCAGTCTGTGGGGATTGAGGGAGGAGCACGGAGGGGCGCTTTCCAGAAGAACAATGACCTAATCCTTTATTACTTCTCAGACGCCAAGACGTTGTATGAAAATTTCCAAAGAGGACTTGCTGTGTCTGACAATGGGCCTTGCTTGGGATACAGAAAGCCAAACCAGCCCTACAAATGGATCTCCTACAAACAGGTGTCTGATCGAGCAGAGTACCTGGGCTCCTGTCTTTTGCATAAAGGATATAAGTCATCACAGGACCAATTTGTTGGCATCTTTGCTCAAAATAGGCCAGAGTGGGTCATCTCTGAGTTAGCCTGTTACACATATTCCATGGTAGCTGTCCCCCTGTATGACACGCTGGGTACAGAAGCCATCATCTTTGTCATCAACAGGGCTGATATCCCTGTGGTCATCTGTGATACACCCCAAAAGGCAACAATGCTAGTAGAAAATGTGGAAAAGGGCCTCACACCAGGCCTGAAGACGATCATCCTCATGGACCCTTTTGATGACGACCTGATGAAAAGAGGAGAAAAGTGTGGAGTTGAGATGTTATCCCTGCATGATGCGGAGAATATAGGCAAAGAGAACTTCAAAAAACCAGTGCCTCCGAAGCCAGAGGATCTGAGCGTCATCTGTTTCACCAGTGGAACTACAGGTGACCCCAAAGGAGCTATGCTAACCCATGAAAATGTTGTTTCAAACATGGCTGCTTTCCTCAAATTTCTGGAGCCTATCTTCCAGCCCACCTCTGATGATGTGACCATATCCTACCTTCCCTTGGCTCATATGTTTGAGAGGCTTGTCCAGGGTATCTTATTTTCCTGTGGAGGCAAAATTGGGTTCTTCCAAGGAGATATCCGGTTGCTACCTGATGACATGAAGGCTTTGAAACCCACAGTGTTTCCTACAGTGCCTCGACTCCTTAACAGGGTCTATGATAAGGTACAAAATGAAGCCAAGACACCTTTGAAGAAGTTCTTGTTGAACTTGGCTATCATCAGTAAATTCAATGAAGTGAAAAATGGCATCATTCGGCGGGACAGTTTGTGGGACAAGCTCGTGTTTTCAAAGATTCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCATTCGGCGGGACAGTTT
60.04
CCAAACGTCAGGAGGCAGAT
60.04
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAATGGCATCATTCGGCGG
59.9
CCAAACGTCAGGAGGCAGAT
60.04
159
PCR template sequence:
CTTGTTGAACTTGGCTATCATCAGTAAATTCAATGAAGTGAAAAATGGCATCATTCGGCGGGACAGTTTGTGGGACAAGCTCGTGTTTTCAAAGATTCAAGTCTGAATTTCCTGTTGGTATTCACAAAGATGCTTTTTATTTTTAACTTCTTGTTTTCCCCACTTCCAACTCCGGCCCTGATCTGCCTCCTGACGTTTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-135a-5p(1), mmu-miR-205-5p(1), mmu-miR-709(1), mmu-miR-143-3p(1), mmu-miR-194-5p(1), mmu-miR-511-3p(1), mmu-miR-383-5p(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCAAAGATTCAAGTCTGAATTTCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACSL5_1247_ORF_1
1.0495
0.194457024
0.998126628
1224
ORF sequence:
ATGCTTTTTATTTTTAACTTCTTGTTTTCCCCACTTCCAACTCCGGCCCTGATCTGCCTCCTGACGTTTGGAACGGCCATCTTCCTGTGGCTGATCAACAGACCTCAGCCAGTTTTACCTCTCATTGACTTGGACAACCAGTCTGTGGGGATTGAGGGAGGAGCACGGAGGGGCGCTTTCCAGAAGAACAATGACCTAATCCTTTATTACTTCTCAGACGCCAAGACGTTGTATGAAAATTTCCAAAGAGGACTTGCTGTGTCTGACAATGGGCCTTGCTTGGGATACAGAAAGCCAAACCAGCCCTACAAATGGATCTCCTACAAACAGGTGTCTGATCGAGCAGAGTACCTGGGCTCCTGTCTTTTGCATAAAGGATATAAGTCATCACAGGACCAATTTGTTGGCATCTTTGCTCAAAATAGGCCAGAGTGGGTCATCTCTGAGTTAGCCTGTTACACATATTCCATGGTAGCTGTCCCCCTGTATGACACGCTGGGTACAGAAGCCATCATCTTTGTCATCAACAGGGCTGATATCCCTGTGGTCATCTGTGATACACCCCAAAAGGCAACAATGCTAGTAGAAAATGTGGAAAAGGGCCTCACACCAGGCCTGAAGACGATCATCCTCATGGACCCTTTTGATGACGACCTGATGAAAAGAGGAGAAAAGTGTGGAGTTGAGATGTTATCCCTGCATGATGCGGAGAATATAGGCAAAGAGAACTTCAAAAAACCAGTGCCTCCGAAGCCAGAGGATCTGAGCGTCATCTGTTTCACCAGTGGAACTACAGGTGACCCCAAAGGAGCTATGCTAACCCATGAAAATGTTGTTTCAAACATGGCTGCTTTCCTCAAATTTCTGGAGCCTATCTTCCAGCCCACCTCTGATGATGTGACCATATCCTACCTTCCCTTGGCTCATATGTTTGAGAGGCTTGTCCAGGGTATCTTATTTTCCTGTGGAGGCAAAATTGGGTTCTTCCAAGGAGATATCCGGTTGCTACCTGATGACATGAAGGCTTTGAAACCCACAGTGTTTCCTACAGTGCCTCGACTCCTTAACAGGGTCTATGATAAGGTACAAAATGAAGCCAAGACACCTTTGAAGAAGTTCTTGTTGAACTTGGCTATCATCAGTAAATTCAATGAAGTGAAAAATGGCATCATTCGGCGGGACAGTTTGTGGGACAAGCTCGTGTTTTCAAAGATTCAAGTCTGA
Peptide sequence:
MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYENFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILMDPFDDDLMKRGEKCGVEMLSLHDAENIGKENFKKPVPPKPEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVKNGIIRRDSLWDKLVFSKIQV
IRESfinder result
IRESfinder score
0.664632966
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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