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mmu_circAcsf2_1347
Basic information of circular RNA
PanCircBase ID:
mmu_circAcsf2_1347
mm39_Chr. Position ID:
chr11|94452065|94464054|-
Gene name:
Acsf2
Transcript:
ENSMUST00000103164.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,1571,8070,8701,9063,9374,10031,10322,10986,11282,11793
Genomic length:
11989
Exon/intron sizes in nt:
15,21,08,77,92,15,89,60,00,00,00,00,00,000
Flanking exon/introns:
chr11:94450430-94452065|chr11:94464054-94492468
Splice length:
1347
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTCTGGAATGGTGAACTGCACCAACCCCTTGCCCATCGGAGGCCTCAGCTACATTCAGGGCCACACCGACTCCCATCTTGTGAACACAACTGTGGGCGAGTGCCTTGACGCCACGGCGCAGAGGTTCCCCGACAGAGAGGCTTTGGTTATCCTCCATGAAAACATCAGGCTGAACTTTGCGCAGCTCAAGGAGGAGGTGGACAAAGCTGCTTCTGGTCTTCTGAGCATTGGCCTCCGCAAGGGTGACCGGCTGGGCATGTGGGGACCCAACTCCTATGCCTGGGTACTTATTCAGCTGGCTACTGCCCAGGCAGGCATCATCCTGGTGTCTGTGAACCCAGCCTACCAGTCCTCAGAACTGGAATATGTCCTCAGAAAGGTAGGCTGCAAAGGTATTGTGTTTCCCAAACAATTCAAGACCCAGCAGTACTACGACATCCTGAAGCAGGTCTGTCCTGAGCTGGAAAAGGCTCAGCCAGGGGCCTTGAAGAGTGAGAGACTCCCAGACCTGACTACAGTCATCTCAGTGGATGCCCCTCTGCCGGGGACCCTGCTCCTGGATGATATTGTGGCAGCCGGTGGCAAGGAACAGAACCTAGCCCAGCTGCGGTACAACCAGCGGTTCTTGTCCTGCTACGACCCCATCAACATCCAGTTCACCTCGGGGACAACTGGAAACCCCAAGGGGGCCACTCTTTCCCACCACAACATCGTAAACAACTCCATGCTGATAGGCCAGCGTCTGAAGATGCCCACAAAGACTGCAGAGGAGTTGCGCTTGGTCCTGCCCAGTCCCCTGTACCACTGCCTGGGCTCCGTGGGGGGCACAATGGTGTCTATGATGCATGGTGCCACTCTCCTCCTGTCTTCTCCAAGCTTCAATGGCAAGAAGGCATTGGAAGCCATCAGCAGAGAGAAAGGCACCCTCCTGTATGGCACACCCACGATGTTTGTGGATATTCTGAATCAGCCAGACTTCTCCAGTTATGATTTCACAAGCATTCGTGGAGGTGTGATTGCTGGGTCCCCAGCACCCCCGGAATTGATTCGAGCCATCATCAACAAGATGAACATGAAGGAGCTGGTGGTTGTTTATGGAACCACAGAGAACAGTCCCGTGACCTTCATGAACTTTCCCGAGGACACCCTGGAGCAGAAGGCTGGTAGTGTGGGCAGAATTATGCCTCACACGGAGGCCCAGATTGTGAATGTGGAGACGGGTGAGCTGACCAACCTGAACGTGCCTGGGGAGCTGTATATCCGAGGGTACTGCGTCATGCAGGGCTACTGGGGTGAGCCACAGAAGACCTTCGAGACGGTCGGACAAGACAAGTGGTATCGGACAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATCCGAGGGTACTGCGTCA
60.11
GTGTGGCCCTGAATGTAGCT
60.04
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TATCCGAGGGTACTGCGTCA
60.11
TGTGGCCCTGAATGTAGCTG
60.04
155
PCR template sequence:
GGGAGCTGTATATCCGAGGGTACTGCGTCATGCAGGGCTACTGGGGTGAGCCACAGAAGACCTTCGAGACGGTCGGACAAGACAAGTGGTATCGGACAGGTTCTGGAATGGTGAACTGCACCAACCCCTTGCCCATCGGAGGCCTCAGCTACATTCAGGGCCACACCGACTCCCATCTTGTGAACACAACTGTGGGCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-680(2), mmu-miR-135a-5p(1), mmu-miR-34c-5p(1), mmu-miR-127-5p(1), mmu-miR-483-5p(1), mmu-miR-34b-5p(1), mmu-miR-1904(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTATCGGACAGGTTCTGGAATGGTG
siRNA sets
CACCATTCCAGAACCTGTC, ACCATTCCAGAACCTGTCC, CCATTCCAGAACCTGTCCG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACSF2_1347_ORF_1
1.2856
0.424628854
0.999999987
2685
ORF sequence:
ATGGTGAACTGCACCAACCCCTTGCCCATCGGAGGCCTCAGCTACATTCAGGGCCACACCGACTCCCATCTTGTGAACACAACTGTGGGCGAGTGCCTTGACGCCACGGCGCAGAGGTTCCCCGACAGAGAGGCTTTGGTTATCCTCCATGAAAACATCAGGCTGAACTTTGCGCAGCTCAAGGAGGAGGTGGACAAAGCTGCTTCTGGTCTTCTGAGCATTGGCCTCCGCAAGGGTGACCGGCTGGGCATGTGGGGACCCAACTCCTATGCCTGGGTACTTATTCAGCTGGCTACTGCCCAGGCAGGCATCATCCTGGTGTCTGTGAACCCAGCCTACCAGTCCTCAGAACTGGAATATGTCCTCAGAAAGGTAGGCTGCAAAGGTATTGTGTTTCCCAAACAATTCAAGACCCAGCAGTACTACGACATCCTGAAGCAGGTCTGTCCTGAGCTGGAAAAGGCTCAGCCAGGGGCCTTGAAGAGTGAGAGACTCCCAGACCTGACTACAGTCATCTCAGTGGATGCCCCTCTGCCGGGGACCCTGCTCCTGGATGATATTGTGGCAGCCGGTGGCAAGGAACAGAACCTAGCCCAGCTGCGGTACAACCAGCGGTTCTTGTCCTGCTACGACCCCATCAACATCCAGTTCACCTCGGGGACAACTGGAAACCCCAAGGGGGCCACTCTTTCCCACCACAACATCGTAAACAACTCCATGCTGATAGGCCAGCGTCTGAAGATGCCCACAAAGACTGCAGAGGAGTTGCGCTTGGTCCTGCCCAGTCCCCTGTACCACTGCCTGGGCTCCGTGGGGGGCACAATGGTGTCTATGATGCATGGTGCCACTCTCCTCCTGTCTTCTCCAAGCTTCAATGGCAAGAAGGCATTGGAAGCCATCAGCAGAGAGAAAGGCACCCTCCTGTATGGCACACCCACGATGTTTGTGGATATTCTGAATCAGCCAGACTTCTCCAGTTATGATTTCACAAGCATTCGTGGAGGTGTGATTGCTGGGTCCCCAGCACCCCCGGAATTGATTCGAGCCATCATCAACAAGATGAACATGAAGGAGCTGGTGGTTGTTTATGGAACCACAGAGAACAGTCCCGTGACCTTCATGAACTTTCCCGAGGACACCCTGGAGCAGAAGGCTGGTAGTGTGGGCAGAATTATGCCTCACACGGAGGCCCAGATTGTGAATGTGGAGACGGGTGAGCTGACCAACCTGAACGTGCCTGGGGAGCTGTATATCCGAGGGTACTGCGTCATGCAGGGCTACTGGGGTGAGCCACAGAAGACCTTCGAGACGGTCGGACAAGACAAGTGGTATCGGACAGGTTCTGGAATGGTGAACTGCACCAACCCCTTGCCCATCGGAGGCCTCAGCTACATTCAGGGCCACACCGACTCCCATCTTGTGAACACAACTGTGGGCGAGTGCCTTGACGCCACGGCGCAGAGGTTCCCCGACAGAGAGGCTTTGGTTATCCTCCATGAAAACATCAGGCTGAACTTTGCGCAGCTCAAGGAGGAGGTGGACAAAGCTGCTTCTGGTCTTCTGAGCATTGGCCTCCGCAAGGGTGACCGGCTGGGCATGTGGGGACCCAACTCCTATGCCTGGGTACTTATTCAGCTGGCTACTGCCCAGGCAGGCATCATCCTGGTGTCTGTGAACCCAGCCTACCAGTCCTCAGAACTGGAATATGTCCTCAGAAAGGTAGGCTGCAAAGGTATTGTGTTTCCCAAACAATTCAAGACCCAGCAGTACTACGACATCCTGAAGCAGGTCTGTCCTGAGCTGGAAAAGGCTCAGCCAGGGGCCTTGAAGAGTGAGAGACTCCCAGACCTGACTACAGTCATCTCAGTGGATGCCCCTCTGCCGGGGACCCTGCTCCTGGATGATATTGTGGCAGCCGGTGGCAAGGAACAGAACCTAGCCCAGCTGCGGTACAACCAGCGGTTCTTGTCCTGCTACGACCCCATCAACATCCAGTTCACCTCGGGGACAACTGGAAACCCCAAGGGGGCCACTCTTTCCCACCACAACATCGTAAACAACTCCATGCTGATAGGCCAGCGTCTGAAGATGCCCACAAAGACTGCAGAGGAGTTGCGCTTGGTCCTGCCCAGTCCCCTGTACCACTGCCTGGGCTCCGTGGGGGGCACAATGGTGTCTATGATGCATGGTGCCACTCTCCTCCTGTCTTCTCCAAGCTTCAATGGCAAGAAGGCATTGGAAGCCATCAGCAGAGAGAAAGGCACCCTCCTGTATGGCACACCCACGATGTTTGTGGATATTCTGAATCAGCCAGACTTCTCCAGTTATGATTTCACAAGCATTCGTGGAGGTGTGATTGCTGGGTCCCCAGCACCCCCGGAATTGATTCGAGCCATCATCAACAAGATGAACATGAAGGAGCTGGTGGTTGTTTATGGAACCACAGAGAACAGTCCCGTGACCTTCATGAACTTTCCCGAGGACACCCTGGAGCAGAAGGCTGGTAGTGTGGGCAGAATTATGCCTCACACGGAGGCCCAGATTGTGAATGTGGAGACGGGTGAGCTGACCAACCTGAACGTGCCTGGGGAGCTGTATATCCGAGGGTACTGCGTCATGCAGGGCTACTGGGGTGAGCCACAGAAGACCTTCGAGACGGTCGGACAAGACAAGTGGTATCGGACA
Peptide sequence:
MVNCTNPLPIGGLSYIQGHTDSHLVNTTVGECLDATAQRFPDREALVILHENIRLNFAQLKEEVDKAASGLLSIGLRKGDRLGMWGPNSYAWVLIQLATAQAGIILVSVNPAYQSSELEYVLRKVGCKGIVFPKQFKTQQYYDILKQVCPELEKAQPGALKSERLPDLTTVISVDAPLPGTLLLDDIVAAGGKEQNLAQLRYNQRFLSCYDPINIQFTSGTTGNPKGATLSHHNIVNNSMLIGQRLKMPTKTAEELRLVLPSPLYHCLGSVGGTMVSMMHGATLLLSSPSFNGKKALEAISREKGTLLYGTPTMFVDILNQPDFSSYDFTSIRGGVIAGSPAPPELIRAIINKMNMKELVVVYGTTENSPVTFMNFPEDTLEQKAGSVGRIMPHTEAQIVNVETGELTNLNVPGELYIRGYCVMQGYWGEPQKTFETVGQDKWYRTGSGMVNCTNPLPIGGLSYIQGHTDSHLVNTTVGECLDATAQRFPDREALVILHENIRLNFAQLKEEVDKAASGLLSIGLRKGDRLGMWGPNSYAWVLIQLATAQAGIILVSVNPAYQSSELEYVLRKVGCKGIVFPKQFKTQQYYDILKQVCPELEKAQPGALKSERLPDLTTVISVDAPLPGTLLLDDIVAAGGKEQNLAQLRYNQRFLSCYDPINIQFTSGTTGNPKGATLSHHNIVNNSMLIGQRLKMPTKTAEELRLVLPSPLYHCLGSVGGTMVSMMHGATLLLSSPSFNGKKALEAISREKGTLLYGTPTMFVDILNQPDFSSYDFTSIRGGVIAGSPAPPELIRAIINKMNMKELVVVYGTTENSPVTFMNFPEDTLEQKAGSVGRIMPHTEAQIVNVETGELTNLNVPGELYIRGYCVMQGYWGEPQKTFETVGQDKWYRT
IRESfinder result
IRESfinder score
0.437789338
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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