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mmu_circAcp2_426
Basic information of circular RNA
PanCircBase ID:
mmu_circAcp2_426
mm39_Chr. Position ID:
chr2|91033945|91036622|+
Gene name:
Acp2
Transcript:
ENSMUST00000150403.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
2,3,4,5
Exon/intron offsets:
0,639,2155,2587
Genomic length:
2677
Exon/intron sizes in nt:
96,87,153,90
Flanking exon/introns:
chr2:91033393-91033945|chr2:91036622-91037061
Splice length:
426
Other database IDs:
CIRCpedia: MMU_CIRCpedia_39908, circAtlas: mmu-Acp2_0001
Human Ortholog IDs:
Mature spliced Sequence:
CTGTATCGACATGGAGATCGGTCACCAGTGAAGACATATCCCAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAACCAAGGAAGGGATGCTACAGCACTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAACACCTCTTACCACAGGCAAGAGGTTTATGTGCGAAGCACAGACTTCGATCGTACTCTCATGAGTGCTGAGGCCAACCTGGCTGGACTCTTCCCTCCCAATGAAGTTCAGCACTTCAACCCTAACATTTCATGGCAGCCTATCCCTGTTCACACTGTGCCCATCACTGAAGACAGGCAATTTCTGAACATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACACACTCTTTTGTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGCTTACAAACGTGACCCT
60.25
AATCCCTGGGGCCATTTCTC
59.74
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGCTTACAAACGTGACCC
60.25
AATCCCTGGGGCCATTTCTC
59.74
141
PCR template sequence:
TGAAGACAGGCAATTTCTGAACATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACACACTCTTTTGTGAGCTGTATCGACATGGAGATCGGTCACCAGTGAAGACATATCCCAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAACCAAGGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-203-3p(1), mmu-miR-190b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTCTTTTGTGAGCTGTATCGACATG
siRNA sets
CATGTCGATACAGCTCACA, GATACAGCTCACAAAAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACP2_426_ORF_1
1.2368
0.327647697
0.977878676
750
ORF sequence:
ATGCTACAGCACTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAACACCTCTTACCACAGGCAAGAGGTTTATGTGCGAAGCACAGACTTCGATCGTACTCTCATGAGTGCTGAGGCCAACCTGGCTGGACTCTTCCCTCCCAATGAAGTTCAGCACTTCAACCCTAACATTTCATGGCAGCCTATCCCTGTTCACACTGTGCCCATCACTGAAGACAGGCAATTTCTGAACATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACACACTCTTTTGTGAGCTGTATCGACATGGAGATCGGTCACCAGTGAAGACATATCCCAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAACCAAGGAAGGGATGCTACAGCACTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAACACCTCTTACCACAGGCAAGAGGTTTATGTGCGAAGCACAGACTTCGATCGTACTCTCATGAGTGCTGAGGCCAACCTGGCTGGACTCTTCCCTCCCAATGAAGTTCAGCACTTCAACCCTAACATTTCATGGCAGCCTATCCCTGTTCACACTGTGCCCATCACTGAAGACAGGCAATTTCTGAACATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACACACTCTTTTGTGAG
Peptide sequence:
MLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRQFLNMVANETGLTNVTLETIWNVYDTLFCELYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRQFLNMVANETGLTNVTLETIWNVYDTLFCE
IRESfinder result
IRESfinder score
0.354362743
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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