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mmu_circAcly_1649
Basic information of circular RNA
PanCircBase ID:
mmu_circAcly_1649
mm39_Chr. Position ID:
chr11|100383330|100407732|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
20,19,18,17,16,15,14,13,12,11,10,9,8,7
Exon/intron offsets:
0,1032,1379,3355,5680,6116,10655,11698,12350,20371,21771,22457,23397,24271
Genomic length:
24402
Exon/intron sizes in nt:
1,11,82,19,71,05,16,91,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:100375553-100383330|chr11:100407732-100409914
Splice length:
1649
Other database IDs:
circAtlas: mmu-Acly_0016
CIRCpedia: HSA_CIRCpedia_353300
Human Ortholog IDs:
Mature spliced Sequence:
TGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAGCCTGGATGCTTTAAGATCGGGAATACTGGTGGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCCCAGGCAGCGTGGCCTACGTCTCACGTTCAGGAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGACCACAGATGGTGTCTATGAGGGCGTGGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCACGTGCTGCGCTACCAGGACACTCCAGGAGTCAAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGGAATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAATATAAGATCTGCCGGGGC
58.37
GTAGTCAGCTGTGGCATCCA
59.75
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAATATAAGATCTGCCGGGGC
58.37
TGTAGTCAGCTGTGGCATCC
59.75
162
PCR template sequence:
AGAGGAATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-339-5p(1), mmu-miR-149-5p(1), mmu-miR-34b-5p(1), mmu-miR-325-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-207(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTCTCCTCCGAGTGGTGACCAAAGA
siRNA sets
TCTTTGGTCACCACTCGGA, TTGGTCACCACTCGGAGGA, TGGTCACCACTCGGAGGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACLY_1649_ORF_1
1.2612
0.462118832
0.999909142
1440
ORF sequence:
ATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAGCCTGGATGCTTTAAGATCGGGAATACTGGTGGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCCCAGGCAGCGTGGCCTACGTCTCACGTTCAGGAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGACCACAGATGGTGTCTATGAGGGCGTGGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCACGTGCTGCGCTACCAGGACACTCCAGGAGTCAAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGGAATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAGTGGTGA
Peptide sequence:
MVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPVVCWCIGTCATMFSSEW
IRESfinder result
IRESfinder score
0.384771055
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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