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mmu_circAcly_1452
Basic information of circular RNA
PanCircBase ID:
mmu_circAcly_1452
mm39_Chr. Position ID:
chr11|100395028|100414447|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,652,8673,10073,10759,11699,12573,14886,15412,16088,17779,19237
Genomic length:
19419
Exon/intron sizes in nt:
9,11,55,11,86,21,37,11,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:100394015-100395028|chr11:100414447-100418620
Splice length:
1452
Other database IDs:
circAtlas: mmu-Acly_0020
Human Ortholog IDs:
Mature spliced Sequence:
GTAAACCAGGTCTCTCTGCAGCCATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCACCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGCCTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCACTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGAGGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTGGATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGGACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGGTCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCGACTCCAGCACCCAGTA
60.32
AGGAGTTCTTTGCCGGTCTG
59.96
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCGACTCCAGCACCCAGTA
60.32
CAGGAGTTCTTTGCCGGTCT
59.96
163
PCR template sequence:
AGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGGTAAACCAGGTCTCTCTGCAGCCATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-143-3p(1), mmu-miR-34b-5p(1), mmu-miR-325-3p(1), mmu-miR-1198-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GCTATGCCCCAAGGTAAACCAGGTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACLY_1452_ORF_1
1.2928
0.445903711
0.999999997
2880
ORF sequence:
ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCACCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGCCTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCACTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGAGGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTGGATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGGACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGGTCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGGTAAACCAGGTCTCTCTGCAGCCATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCACCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGCCTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCACTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGAGGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTGGATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGGACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGGTCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAA
Peptide sequence:
MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLGLVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNTEDIKRHLLVHAPEDKKEVLASFISGLFNFYEDLYFTYLEINPLVVTKDGVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQGKPGLSAAMSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLGLVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNTEDIKRHLLVHAPEDKKEVLASFISGLFNFYEDLYFTYLEINPLVVTKDGVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQ
IRESfinder result
IRESfinder score
0.392511903
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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