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mmu_circAcly_1009
Basic information of circular RNA
PanCircBase ID:
mmu_circAcly_1009
mm39_Chr. Position ID:
chr11|100386685|100405924|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
17,16,15,14,13,12,11,10,9
Exon/intron offsets:
0,2325,2761,7300,8343,8995,17016,18416,19102
Genomic length:
19239
Exon/intron sizes in nt:
1,05,16,91,42,30,91,15,00,00,00,000
Flanking exon/introns:
chr11:100384906-100386685|chr11:100405924-100406727
Splice length:
1009
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAAGGTATCCCTGAGGC
59.89
AGGTCTGTTGTTCACTGGGG
59.53
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAAGGTATCCCTGAGGC
59.89
GGTCTGTTGTTCACTGGGGG
60.54
166
PCR template sequence:
CACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-149-5p(1), mmu-miR-34b-5p(1), mmu-miR-325-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-207(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGGCCAGCTACGTGACACCATCTGT
siRNA sets
ACAGATGGTGTCACGTAGC, GATGGTGTCACGTAGCTGG, CAGATGGTGTCACGTAGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACLY_1009_ORF_1
1.2437
0.472124005
0.995711732
900
ORF sequence:
ATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGTGA
Peptide sequence:
MTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPAT
IRESfinder result
IRESfinder score
0.390912845
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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