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mmu_circAcer3_341
Basic information of circular RNA
PanCircBase ID:
mmu_circAcer3_341
mm39_Chr. Position ID:
chr7|97879656|97906984|-
Gene name:
Acer3
Transcript:
ENSMUST00000151258.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,13499,21440,27246
Genomic length:
27328
Exon/intron sizes in nt:
164,59,36,82
Flanking exon/introns:
chr7:97875707-97879656|chr7:97906984-97908611
Splice length:
341
Other database IDs:
circBase: mmu_circ_0013681, CIRCpedia: MMU_CIRCpedia_35755, circAtlas: mmu-Acer3_0018
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAAGAACCTATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACATGTGTATCTCCAGAGTCTTGTCTGTACTGAGCATGAATGGACCATGTGCTGATGAAGTTCTTGCTCTCTCCTTCGCGCCCTGCTACCTGGATAACCAAAGACATTCATTTCACCCTACTGAAGAATTTTGCTTTGTACTTCTTCTGAGAACTGAAGATTTGCCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCTGCTACCTGGATAACC
59.89
AGGGTAAAAAGAAGATGGTAGTT
55.21
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCCCTGCTACCTGGATAAC
60.25
AGGGTAAAAAGAAGATGGTAGTT
55.21
145
PCR template sequence:
TCTCCTTCGCGCCCTGCTACCTGGATAACCAAAGACATTCATTTCACCCTACTGAAGAATTTTGCTTTGTACTTCTTCTGAGAACTGAAGATTTGCCATGGTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-185-5p(1), mmu-miR-325-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGATTTGCCATGGTTTGAGTGTTTC
siRNA sets
GAAACACTCAAACCATGGC,AACACTCAAACCATGGCAA,AAACACTCAAACCATGGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACER3_341_ORF_1
0.4528
-0.216800038
0.06792759
372
ORF sequence:
ATGTGTATCTCCAGAGTCTTGTCTGTACTGAGCATGAATGGACCATGTGCTGATGAAGTTCTTGCTCTCTCCTTCGCGCCCTGCTACCTGGATAACCAAAGACATTCATTTCACCCTACTGAAGAATTTTGCTTTGTACTTCTTCTGAGAACTGAAGATTTGCCATGGTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAAGAACCTATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACATGTGTATCTCCAGAGTCTTGTCTGTACTGA
Peptide sequence:
MCISRVLSVLSMNGPCADEVLALSFAPCYLDNQRHSFHPTEEFCFVLLLRTEDLPWFECFKTKSSINYHLLFTLFLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTCVSPESCLY
IRESfinder result
IRESfinder score
0.935510193
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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