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mmu_circAcer3_177
Basic information of circular RNA
PanCircBase ID:
mmu_circAcer3_177
mm39_Chr. Position ID:
chr7|97893155|97906984|-
Gene name:
Acer3
Transcript:
ENSMUST00000033020.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,6,5
Exon/intron offsets:
0,7941,13747
Genomic length:
13829
Exon/intron sizes in nt:
59,36,82
Flanking exon/introns:
chr7:97875707-97893155|chr7:97906984-97908611
Splice length:
177
Other database IDs:
circBase: mmu_circ_0013682, CIRCpedia: MMU_CIRCpedia_35760, circAtlas: mmu-Acer3_0009
circBase: hsa_circ_0023670, circAtlas: hsa-ACER3_0010, CIRCpedia: HSA_CIRCpedia_5680
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAAGAACCTATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCATCAGGTCATGTATGGAATGT
59.59
AGGGTAAAAAGAAGATGGTAGTT
55.21
121
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCATCAGGTCATGTATGGAATGT
59.59
AGGGTAAAAAGAAGATGGTAGTTT
55.94
121
PCR template sequence:
TAAAAGTCAAAGAACCTATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACATGGTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-325-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATATTGTTACATGGTTTGAGTGTTTC
siRNA sets
AAACACTCAAACCATGTAA, AACACTCAAACCATGTAAC, ACACTCAAACCATGTAACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACER3_177_ORF_1
0.7526
-0.305657051
0.031232908
231
ORF sequence:
ATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACATGGTTTGAGTGTTTCAAGACAAAGAGCTCAATAAACTACCATCTTCTTTTTACCCTATTTCTATACAGTTTAACAGTAACTACGATTTACCTAAAAGTCAAAGAACCTATATTCCATCAGGTCATGTATGGAATGTTGGTCTTTACATTAGTACTTCGTTCTATTTATATTGTTACA
Peptide sequence:
MYGMLVFTLVLRSIYIVTWFECFKTKSSINYHLLFTLFLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLRSIYIVT
IRESfinder result
IRESfinder score
0.967422318
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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