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mmu_circAce2_1398
Basic information of circular RNA
PanCircBase ID:
mmu_circAce2_1398
mm39_Chr. Position ID:
chrX|162940853|162958981|+
Gene name:
Ace2
Transcript:
ENSMUST00000112271.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
5,6,7,8,9,10,11,12,13,14
Exon/intron offsets:
0,2906,4423,6067,9947,11726,14511,16217,17404,17955
Genomic length:
18128
Exon/intron sizes in nt:
1,44,11,31,06,98,17,02,00,00,00,00,00,000
Flanking exon/introns:
chrX:162939596-162940853|chrX:162958981-162960473
Splice length:
1398
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATTGGATGAAATAATGGCGACAAGCACAGACTACAACTCTAGGCTCTGGGCATGGGAGGGCTGGAGGGCTGAGGTTGGCAAGCAGCTGAGGCCGTTGTATGAAGAGTATGTGGTCCTGAAAAACGAGATGGCAAGAGCAAACAATTATAACGACTATGGGGATTATTGGAGAGGGGACTATGAAGCAGAGGGAGCAGATGGCTACAACTATAACCGTAACCAGTTGATTGAAGATGTAGAACGTACCTTCGCAGAGATCAAGCCATTGTATGAGCATCTTCATGCCTATGTGAGGAGGAAGTTGATGGATACCTACCCTTCCTACATCAGCCCCACTGGATGCCTCCCTGCCCATTTGCTTGGTGATATGTGGGGTAGATTTTGGACAAATCTGTACCCTTTGACTGTTCCCTTTGCACAGAAACCAAACATAGATGTTACTGATGCAATGATGAATCAGGGCTGGGATGCAGAAAGGATATTTCAAGAGGCAGAGAAATTCTTTGTTTCTGTTGGCCTTCCTCATATGACTCAAGGATTCTGGGCAAACTCTATGCTGACTGAGCCAGCAGATGGCCGGAAAGTTGTCTGCCACCCCACAGCTTGGGATCTGGGACACGGAGACTTCAGAATCAAGATGTGTACAAAGGTCACAATGGACAACTTCTTGACAGCCCATCACGAGATGGGACACATCCAATATGACATGGCATATGCCAGGCAACCTTTCCTGCTAAGAAACGGAGCCAATGAAGGGTTCCATGAAGCTGTTGGAGAAATCATGTCACTTTCTGCAGCTACCCCCAAGCATCTGAAATCCATTGGTCTTCTGCCATCCGATTTTCAAGAAGATAGCGAAACAGAGATAAACTTCCTACTGAAACAGGCATTGACAATTGTTGGAACACTACCGTTTACTTACATGTTAGAGAAGTGGAGGTGGATGGTCTTTCGGGGTGAAATTCCCAAAGAGCAGTGGATGAAAAAGTGGTGGGAGATGAAGCGGGAGATCGTTGGTGTGGTGGAGCCTCTGCCTCATGATGAAACATACTGTGACCCTGCATCTCTGTTCCATGTTTCTAATGATTACTCATTCATTCGATATTACACAAGGACCATTTACCAATTCCAGTTTCAAGAAGCTCTTTGTCAAGCAGCTAAGTATAATGGTTCTCTGCACAAATGTGACATCTCAAATTCCACTGAAGCTGGGCAGAAGTTGCTCAAGATGCTGAGTCTTGGAAATTCAGAGCCCTGGACCAAAGCCTTGGAAAATGTGGTAGGAGCAAGGAATATGGATGTAAAACCACTGCTCAATTACTTCCAACCGTTGTTTGACTGGCTGAAAGAGCAGAACAGAAATTCTTTTGTGGGGTGGAACACTGAATGGAGCCCAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGTTGTTTGACTGGCTGA
60.11
CTCAGCTGCTTGCCAACCTC
61.58
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGTTGTTTGACTGGCTGA
60.11
TCAGCTGCTTGCCAACCTC
60.6
161
PCR template sequence:
GATGTAAAACCACTGCTCAATTACTTCCAACCGTTGTTTGACTGGCTGAAAGAGCAGAACAGAAATTCTTTTGTGGGGTGGAACACTGAATGGAGCCCATGATTGGATGAAATAATGGCGACAAGCACAGACTACAACTCTAGGCTCTGGGCATGGGAGGGCTGGAGGGCTGAGGTTGGCAAGCAGCTGAGGCCGTTGTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-23a-3p(2), mmu-miR-23b-3p(1), mmu-miR-140-5p(1), mmu-miR-205-5p(1), mmu-miR-7a-5p(1), mmu-miR-709(1), mmu-miR-24-3p(1), mmu-miR-149-5p(1), mmu-miR-218-5p(1), mmu-miR-382-5p(1), mmu-miR-758-3p(1), mmu-miR-362-5p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GAATGGAGCCCATGATTGGATGAAAT
siRNA sets
ATTTCATCCAATCATGGGC, TCATCCAATCATGGGCTCC, TTCATCCAATCATGGGCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACE2_1398_ORF_1
0.7898
0.129616605
0.998760808
1386
ORF sequence:
ATGGCGACAAGCACAGACTACAACTCTAGGCTCTGGGCATGGGAGGGCTGGAGGGCTGAGGTTGGCAAGCAGCTGAGGCCGTTGTATGAAGAGTATGTGGTCCTGAAAAACGAGATGGCAAGAGCAAACAATTATAACGACTATGGGGATTATTGGAGAGGGGACTATGAAGCAGAGGGAGCAGATGGCTACAACTATAACCGTAACCAGTTGATTGAAGATGTAGAACGTACCTTCGCAGAGATCAAGCCATTGTATGAGCATCTTCATGCCTATGTGAGGAGGAAGTTGATGGATACCTACCCTTCCTACATCAGCCCCACTGGATGCCTCCCTGCCCATTTGCTTGGTGATATGTGGGGTAGATTTTGGACAAATCTGTACCCTTTGACTGTTCCCTTTGCACAGAAACCAAACATAGATGTTACTGATGCAATGATGAATCAGGGCTGGGATGCAGAAAGGATATTTCAAGAGGCAGAGAAATTCTTTGTTTCTGTTGGCCTTCCTCATATGACTCAAGGATTCTGGGCAAACTCTATGCTGACTGAGCCAGCAGATGGCCGGAAAGTTGTCTGCCACCCCACAGCTTGGGATCTGGGACACGGAGACTTCAGAATCAAGATGTGTACAAAGGTCACAATGGACAACTTCTTGACAGCCCATCACGAGATGGGACACATCCAATATGACATGGCATATGCCAGGCAACCTTTCCTGCTAAGAAACGGAGCCAATGAAGGGTTCCATGAAGCTGTTGGAGAAATCATGTCACTTTCTGCAGCTACCCCCAAGCATCTGAAATCCATTGGTCTTCTGCCATCCGATTTTCAAGAAGATAGCGAAACAGAGATAAACTTCCTACTGAAACAGGCATTGACAATTGTTGGAACACTACCGTTTACTTACATGTTAGAGAAGTGGAGGTGGATGGTCTTTCGGGGTGAAATTCCCAAAGAGCAGTGGATGAAAAAGTGGTGGGAGATGAAGCGGGAGATCGTTGGTGTGGTGGAGCCTCTGCCTCATGATGAAACATACTGTGACCCTGCATCTCTGTTCCATGTTTCTAATGATTACTCATTCATTCGATATTACACAAGGACCATTTACCAATTCCAGTTTCAAGAAGCTCTTTGTCAAGCAGCTAAGTATAATGGTTCTCTGCACAAATGTGACATCTCAAATTCCACTGAAGCTGGGCAGAAGTTGCTCAAGATGCTGAGTCTTGGAAATTCAGAGCCCTGGACCAAAGCCTTGGAAAATGTGGTAGGAGCAAGGAATATGGATGTAAAACCACTGCTCAATTACTTCCAACCGTTGTTTGACTGGCTGAAAGAGCAGAACAGAAATTCTTTTGTGGGGTGGAACACTGAATGGAGCCCATGA
Peptide sequence:
MATSTDYNSRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYNDYGDYWRGDYEAEGADGYNYNRNQLIEDVERTFAEIKPLYEHLHAYVRRKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFAQKPNIDVTDAMMNQGWDAERIFQEAEKFFVSVGLPHMTQGFWANSMLTEPADGRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYARQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSDFQEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFRGEIPKEQWMKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKYNGSLHKCDISNSTEAGQKLLKMLSLGNSEPWTKALENVVGARNMDVKPLLNYFQPLFDWLKEQNRNSFVGWNTEWSP
IRESfinder result
IRESfinder score
0.473272111
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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