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mmu_circAcbd5_669
Basic information of circular RNA
PanCircBase ID:
mmu_circAcbd5_669
mm39_Chr. Position ID:
chr2|22977496|22984529|+
Gene name:
Acbd5
Transcript:
ENSMUST00000227663.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,8,9,10
Exon/intron offsets:
0,2106,2991,6804
Genomic length:
7033
Exon/intron sizes in nt:
1,35,19,81,07,229
Flanking exon/introns:
chr2:22970712-22977496|chr2:22984529-22989576
Splice length:
669
Other database IDs:
CIRCpedia: MMU_CIRCpedia_13675, circAtlas: mmu-Acbd5_0009
circAtlas: hsa-ACBD5_0021
Human Ortholog IDs:
Mature spliced Sequence:
ATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGAAGAGTTAAAAGGAGCAGAACAGAGCGGCAGTGATGATAAGAAAACGCTGAAGAAATCAGCAGATAAGAATTTGGAAATCATTGTTACCAATGGCTATAAAGGTAGCTTTGTTCAGGATATACAGAGTGACATTCATACTGATTCTTCCCGGAGCACCAGAAGCAGTGAAGATGAAAAGCCTGGAGATGAGAGCTCTCAGCAGACAGGACACACCATTGTCTGTGCTCACCAAGATAGAAATGAAGATCCCAGTGAAGATGCTTCAGGAATACACCATTTAACAAGTGATTCAGATAGTGAAGTGTACTGTGACTCTATGGAGCAATTTGGACAAGAAGAGTATTATTTAGGTGGTGATCCTACTCAGCACTTGGAAAGTTCTGGTTTTTGTGAAGATGCTCAGCAATCTCCTGGAAATGGCAGCATTGGGAAAATGTGGATGGTAGCAGTCAAAGGAAAAGGCGAAGTGAAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGAGAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAAGTAGCAGTGGAGCA
60.04
GCCCCACTATCACTGCTCTC
59.89
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAAGTAGCAGTGGAGCA
60.04
TCTTCAGACTCAGCCCCACT
60.18
166
PCR template sequence:
AAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGAGAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGAGATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-101a-3p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-3470b(1), mmu-miR-350-3p(1), mmu-miR-1904(1), mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGAGAGGGAGAGATCTTGGTAATGT
siRNA sets
ATTACCAAGATCTCTCCCT,ACATTACCAAGATCTCTCC,TTACCAAGATCTCTCCCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACBD5_669_ORF_1
0.9916
0.170882623
0.98044046
921
ORF sequence:
ATGGAGCAATTTGGACAAGAAGAGTATTATTTAGGTGGTGATCCTACTCAGCACTTGGAAAGTTCTGGTTTTTGTGAAGATGCTCAGCAATCTCCTGGAAATGGCAGCATTGGGAAAATGTGGATGGTAGCAGTCAAAGGAAAAGGCGAAGTGAAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGAGAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGAGATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGAAGAGTTAAAAGGAGCAGAACAGAGCGGCAGTGATGATAAGAAAACGCTGAAGAAATCAGCAGATAAGAATTTGGAAATCATTGTTACCAATGGCTATAAAGGTAGCTTTGTTCAGGATATACAGAGTGACATTCATACTGATTCTTCCCGGAGCACCAGAAGCAGTGAAGATGAAAAGCCTGGAGATGAGAGCTCTCAGCAGACAGGACACACCATTGTCTGTGCTCACCAAGATAGAAATGAAGATCCCAGTGAAGATGCTTCAGGAATACACCATTTAACAAGTGATTCAGATAGTGAAGTGTACTGTGACTCTATGGAGCAATTTGGACAAGAAGAGTATTATTTAGGTGGTGATCCTACTCAGCACTTGGAAAGTTCTGGTTTTTGTGAAGATGCTCAGCAATCTCCTGGAAATGGCAGCATTGGGAAAATGTGGATGGTAGCAGTCAAAGGAAAAGGCGAAGTGAAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGAGAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGA
Peptide sequence:
MEQFGQEEYYLGGDPTQHLESSGFCEDAQQSPGNGSIGKMWMVAVKGKGEVKHGGEDGRSSSGAPHRETRGGESEDFSSVRRGRDLGNVLTSSNAKAVNGKAESSDSGAESEEEEAQEELKGAEQSGSDDKKTLKKSADKNLEIIVTNGYKGSFVQDIQSDIHTDSSRSTRSSEDEKPGDESSQQTGHTIVCAHQDRNEDPSEDASGIHHLTSDSDSEVYCDSMEQFGQEEYYLGGDPTQHLESSGFCEDAQQSPGNGSIGKMWMVAVKGKGEVKHGGEDGRSSSGAPHRETRGGESEDFSSVRRGR
IRESfinder result
IRESfinder score
0.446261682
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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