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mmu_circAcbd5_440
Basic information of circular RNA
PanCircBase ID:
mmu_circAcbd5_440
mm39_Chr. Position ID:
chr2|22977496|22980594|+
Gene name:
Acbd5
Transcript:
ENSMUST00000227663.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,8,9
Exon/intron offsets:
0,2106,2991
Genomic length:
3098
Exon/intron sizes in nt:
135,198,107
Flanking exon/introns:
chr2:22970712-22977496|chr2:22980594-22984300
Splice length:
440
Other database IDs:
CIRCpedia: MMU_CIRCpedia_214529, circAtlas: mmu-Acbd5_0002
circAtlas: hsa-ACBD5_0002, CIRCpedia: HSA_CIRCpedia_128880
Human Ortholog IDs:
Mature spliced Sequence:
ATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGAAGAGTTAAAAGGAGCAGAACAGAGCGGCAGTGATGATAAGAAAACGCTGAAGAAATCAGCAGATAAGAATTTGGAAATCATTGTTACCAATGGCTATAAAGGTAGCTTTGTTCAGGATATACAGAGTGACATTCATACTGATTCTTCCCGGAGCACCAGAAGCAGTGAAGATGAAAAGCCTGGAGATGAGAGCTCTCAGCAGACAGGACACACCATTGTCTGTGCTCACCAAGATAGAAATGAAGATCCCAGTGAAGATGCTTCAGGAATACACCATTTAACAAGTGATTCAGATAGTGAAGTGTACTGTGACTCTATGGAGCAATTTGGACAAGAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCAGTGAAGATGCTTCAGGA
59.72
GCCCCACTATCACTGCTCTC
59.89
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCAGTGAAGATGCTTCAGG
59.72
GCCCCACTATCACTGCTCTC
59.89
167
PCR template sequence:
TGAAGATCCCAGTGAAGATGCTTCAGGAATACACCATTTAACAAGTGATTCAGATAGTGAAGTGTACTGTGACTCTATGGAGCAATTTGGACAAGAAGAGATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-101a-3p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-3470b(1), mmu-miR-1904(1), mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGACAAGAAGAGATCTTGGTAATGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.562432741
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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