top of page
mmu_circAcap2_640
Basic information of circular RNA
PanCircBase ID:
mmu_circAcap2_640
mm39_Chr. Position ID:
chr16|30946138|30972577|-
Gene name:
Acap2
Transcript:
ENSMUST00000230698.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
10,9,8,7,6,5,4
Exon/intron offsets:
0,3939,6208,9216,12150,14418,26371
Genomic length:
26439
Exon/intron sizes in nt:
113,75,96,45,184,59,68
Flanking exon/introns:
chr16:30938265-30946138|chr16:30972577-30973390
Splice length:
640
Other database IDs:
CIRCpedia: MMU_CIRCpedia_215730, circAtlas: mmu-Acap2_0029
Human Ortholog IDs:
Mature spliced Sequence:
ACAAGTTTGACCAAGTTTTCTGACAGTCTTCAGGAAATGATCAATTTCCACACAGTAAGTATGTGTGAATTCTGTTTGACCAAACCCAGAGATCAATTAAGGCCCAGCTTCAGAACTTTGTCAAAGAAGATCTTAGAAAATTCAAAGATGCCAAGAAACAATTTGAAAAAGTCAGTGAAGAAAAGGAAAACGCACTAGTAAAAAATGCCCAAGTTCAAAGAAACAAACAGCACGAGGTTGAAGAGGCTGCAAACATTCTCACGGCCACGCGGAAATGCTTCCGGCACATAGCGCTTGACTACGTCCTGCAGATCAATGTGCTTCAGTCAAAACGGAGATCGGAAATCCTGAAATCAATGCTGTCCTTCATGTACGCACATCTGGCCTTCTTCCATCAAGGGTATGACCTGTTCAGTGAGCTTGGGCCCTACATGAAAGATCTTGGAGCACAGTTGGATCGACTGGTTGTAGATGCAGCAAAGGAGAAAAGAGAGATGGAGCAAAAACACTCTACGATCCAGCAGAAGGATTTCTCCAGTGACGATTCCAAGCTAGAGTATAATGTAGACGCTGCAAATGGCATTGTCATGGAAGGGTATCTGTTCAAACGAGCCAGCAACGCCTTCAAAACGTGGAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCATTGTCATGGAAGGGT
59.59
TGATCTCTGGGTTTGGTCAA
56.36
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCATTGTCATGGAAGGGT
59.59
TTGATCTCTGGGTTTGGTCA
56.36
159
PCR template sequence:
ACGATTCCAAGCTAGAGTATAATGTAGACGCTGCAAATGGCATTGTCATGGAAGGGTATCTGTTCAAACGAGCCAGCAACGCCTTCAAAACGTGGAACAGACAAGTTTGACCAAGTTTTCTGACAGTCTTCAGGAAATGATCAATTTCCACACAGTAAGTATGTGTGAATTCTGTTTGACCAAACCCAGAGATCAATTAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAACGTGGAACAGACAAGTTTGACCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACAP2_640_ORF_1
1.0858
0.315575976
0.539423399
294
ORF sequence:
ATGCTGTCCTTCATGTACGCACATCTGGCCTTCTTCCATCAAGGGTATGACCTGTTCAGTGAGCTTGGGCCCTACATGAAAGATCTTGGAGCACAGTTGGATCGACTGGTTGTAGATGCAGCAAAGGAGAAAAGAGAGATGGAGCAAAAACACTCTACGATCCAGCAGAAGGATTTCTCCAGTGACGATTCCAAGCTAGAGTATAATGTAGACGCTGCAAATGGCATTGTCATGGAAGGGTATCTGTTCAAACGAGCCAGCAACGCCTTCAAAACGTGGAACAGACAAGTTTGA
Peptide sequence:
MLSFMYAHLAFFHQGYDLFSELGPYMKDLGAQLDRLVVDAAKEKREMEQKHSTIQQKDFSSDDSKLEYNVDAANGIVMEGYLFKRASNAFKTWNRQV
IRESfinder result
IRESfinder score
0.393549723
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page