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mmu_circAcad8_596
Basic information of circular RNA
PanCircBase ID:
mmu_circAcad8_596
mm39_Chr. Position ID:
chr9|26896758|26903556|-
Gene name:
Acad8
Transcript:
ENSMUST00000120367.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
6,5,4,3,2
Exon/intron offsets:
0,4657,5116,5777,6697
Genomic length:
6798
Exon/intron sizes in nt:
1,38,73,11,41,70,101
Flanking exon/introns:
chr9:26890607-26896758|chr9:26903556-26910689
Splice length:
596
Other database IDs:
CIRCpedia: MMU_CIRCpedia_215787, circAtlas: mmu-Acad8_0001
circBase: hsa_circ_0095381, circAtlas: hsa-ACAD8_0001, CIRCpedia: HSA_CIRCpedia_3785
Human Ortholog IDs:
Mature spliced Sequence:
CTTCCTTGGGGCTAAATGAAGAACAGAAAGGATTTCAGAAAGTGGCCTTTGACTTTGCAGCTCGGGAAATGGCTCCCAATATGGCGGAGTGGGATCAGAAGGAGCTGTTCCCTGTGGATGTGATGCGGAAGGCTGCACAGCTGGGATTTGGGGGGGTCTATGTGCGAACAGATGTGGGTGGATCTGGACTGTCTCGCCTTGATACTTCTGTCATCTTTGAAGCCTTGGCCACAGGCTGCACCAGCACCACTGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTTGGAAATGAGGAACAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTACTGCCTCACTGAACCAGGTGCTGGGAGTGATGCTGCATCTCTTCTGACCTCAGCTAAACAACAAGGAGATCATTACATCCTCAATGGTTCCAAGGCCTTCATCAGTGGGGGAGGTGAGTCAGACATCTATGTGGTCATGTGCCGAACTGGTGAGTCTGGCGCCAAAGGTATCTCCTGCATAGTTGTTGAGAAAGGAACCCCTGGTCTCAGCTTTGGCAAGAAGGAGAAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTGGCGCCAAAGGTATCT
60.11
GAGCTGCAAAGTCAAAGGCC
60.04
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCTGGCGCCAAAGGTATC
60.11
GAGCTGCAAAGTCAAAGGCC
60.04
143
PCR template sequence:
GGTCATGTGCCGAACTGGTGAGTCTGGCGCCAAAGGTATCTCCTGCATAGTTGTTGAGAAAGGAACCCCTGGTCTCAGCTTTGGCAAGAAGGAGAAGAAGCTTCCTTGGGGCTAAATGAAGAACAGAAAGGATTTCAGAAAGTGGCCTTTGACTTTGCAGCTCGGGAAATGGCTCCCAATATGGCGGAGTGGGATCAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-let-7a-5p(1), mmu-miR-103-3p(1), mmu-miR-34a-5p(1), mmu-miR-216a-5p(1), mmu-miR-709(1), mmu-miR-320-3p(1), mmu-miR-138-5p(1), mmu-let-7d-5p(1), mmu-miR-107-3p(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGGAGAAGAAGCTTCCTTGGGGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACAD8_596_ORF_1
1.0581
0.25175946
0.838148357
543
ORF sequence:
ATGGCTCCCAATATGGCGGAGTGGGATCAGAAGGAGCTGTTCCCTGTGGATGTGATGCGGAAGGCTGCACAGCTGGGATTTGGGGGGGTCTATGTGCGAACAGATGTGGGTGGATCTGGACTGTCTCGCCTTGATACTTCTGTCATCTTTGAAGCCTTGGCCACAGGCTGCACCAGCACCACTGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTTGGAAATGAGGAACAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTACTGCCTCACTGAACCAGGTGCTGGGAGTGATGCTGCATCTCTTCTGACCTCAGCTAAACAACAAGGAGATCATTACATCCTCAATGGTTCCAAGGCCTTCATCAGTGGGGGAGGTGAGTCAGACATCTATGTGGTCATGTGCCGAACTGGTGAGTCTGGCGCCAAAGGTATCTCCTGCATAGTTGTTGAGAAAGGAACCCCTGGTCTCAGCTTTGGCAAGAAGGAGAAGAAGCTTCCTTGGGGCTAA
Peptide sequence:
MAPNMAEWDQKELFPVDVMRKAAQLGFGGVYVRTDVGGSGLSRLDTSVIFEALATGCTSTTAYISIHNMCAWMIDSFGNEEQRHKFCPPLCTMEKFASYCLTEPGAGSDAASLLTSAKQQGDHYILNGSKAFISGGGESDIYVVMCRTGESGAKGISCIVVEKGTPGLSFGKKEKKLPWG
IRESfinder result
IRESfinder score
0.575119264
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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