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mmu_circAcaca_980
Basic information of circular RNA
PanCircBase ID:
mmu_circAcaca_980
mm39_Chr. Position ID:
chr11|84134103|84148147|+
Gene name:
Acaca
Transcript:
ENSMUST00000103201.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
12,13,14,15,16,17,18
Exon/intron offsets:
0,1929,2416,6136,7954,13406,13898
Genomic length:
14044
Exon/intron sizes in nt:
1,71,16,21,64,15,11,04,00,000
Flanking exon/introns:
chr11:84129861-84134103|chr11:84148147-84150148
Splice length:
980
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGTGCAGTGAAACTTGCCAAAATGGTTGGTTATGTGAGTGCTGGGACTGTGGAATACTTGTACAGCCAGGATGGAAGCTTCTACTTTTTGGAACTGAACCCTCGGCTACAGGTTGAACATCCTTGTACAGAGATGGTGGCTGATGTCAATCTTCCTGCAGCACAGCTCCAGATTGCCATGGGGATCCCTCTATTTAGGATCAAGGATATTCGTATGATGTATGGGGTATCTCCTTGGGGAGATGCTCCCATTGATTTTGAAAATTCTGCTCATGTTCCTTGCCCAAGGGGCCACGTGATTGCTGCTCGGATCACCAGTGAAAACCCAGATGAGGGGTTTAAGCCCAGCTCTGGAACAGTTCAGGAACTTAATTTTCGTAGCAATAAGAACGTTTGGGGTTATTTCAGTGTTGCTGCTGCTGGAGGACTTCATGAATTTGCTGATTCTCAGTTCGGGCACTGCTTTTCCTGGGGAGAAAACAGGGAGGAAGCAATCTCAAATATGGTGGTGGCACTGAAGGAGCTGTCTATTCGGGGTGACTTTCGAACTACAGTGGAATACCTCATCAAACTGCTGGAGACAGAAAGCTTTCAGCTTAACAGAATCGACACTGGCTGGCTGGACAGACTGATCGCAGAGAAAGTGCAGGCAGAGCGACCTGACACCATGTTGGGAGTTGTGTGTGGGGCTCTCCATGTAGCAGATGTGAGCCTGAGGAACAGCATCTCTAACTTCCTTCACTCCTTAGAGAGGGGTCAAGTCCTTCCTGCTCACACACTTCTGAACACAGTAGATGTTGAACTTATCTATGAAGGAATCAAATATGTACTTAAGGTGACTCGGCAGTCTCCCAACTCCTACGTAGTGATAATGAATGGCTCGTGTGTGGAAGTGGATGTGCATCGGCTGAGTGATGGTGGCCTGCTCTTGTCTTATGACGGCAGCAGTTACACCACATACATGAAGGAAGAGGTGGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGTGCATCGGCTGAGTGAT
59.82
GAAGCTTCCATCCTGGCTGT
60.03
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGTGGAAGTGGATGTGCA
59.82
GAAGCTTCCATCCTGGCTGT
60.03
179
PCR template sequence:
CGTGTGTGGAAGTGGATGTGCATCGGCTGAGTGATGGTGGCCTGCTCTTGTCTTATGACGGCAGCAGTTACACCACATACATGAAGGAAGAGGTGGACAGTGTGCAGTGAAACTTGCCAAAATGGTTGGTTATGTGAGTGCTGGGACTGTGGAATACTTGTACAGCCAGGATGGAAGCTTCTACTTTTTGGAACTGAACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-15b-5p(2), mmu-miR-137-3p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-150-5p(1), mmu-miR-34a-5p(1), mmu-miR-322-5p(1), mmu-miR-182-5p(1), mmu-miR-541-5p(1), mmu-miR-362-5p(1), mmu-miR-297a-5p(1), mmu-miR-27a-5p(1), mmu-miR-342-3p(1), mmu-miR-1187(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
AAGAGGTGGACAGTGTGCAGTGAAAC
siRNA sets
TTTCACTGCACACTGTCCA, TTCACTGCACACTGTCCAC, GTTTCACTGCACACTGTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACACA_980_ORF_1
1.1474
0.20101748
0.990616205
969
ORF sequence:
ATGGTTGGTTATGTGAGTGCTGGGACTGTGGAATACTTGTACAGCCAGGATGGAAGCTTCTACTTTTTGGAACTGAACCCTCGGCTACAGGTTGAACATCCTTGTACAGAGATGGTGGCTGATGTCAATCTTCCTGCAGCACAGCTCCAGATTGCCATGGGGATCCCTCTATTTAGGATCAAGGATATTCGTATGATGTATGGGGTATCTCCTTGGGGAGATGCTCCCATTGATTTTGAAAATTCTGCTCATGTTCCTTGCCCAAGGGGCCACGTGATTGCTGCTCGGATCACCAGTGAAAACCCAGATGAGGGGTTTAAGCCCAGCTCTGGAACAGTTCAGGAACTTAATTTTCGTAGCAATAAGAACGTTTGGGGTTATTTCAGTGTTGCTGCTGCTGGAGGACTTCATGAATTTGCTGATTCTCAGTTCGGGCACTGCTTTTCCTGGGGAGAAAACAGGGAGGAAGCAATCTCAAATATGGTGGTGGCACTGAAGGAGCTGTCTATTCGGGGTGACTTTCGAACTACAGTGGAATACCTCATCAAACTGCTGGAGACAGAAAGCTTTCAGCTTAACAGAATCGACACTGGCTGGCTGGACAGACTGATCGCAGAGAAAGTGCAGGCAGAGCGACCTGACACCATGTTGGGAGTTGTGTGTGGGGCTCTCCATGTAGCAGATGTGAGCCTGAGGAACAGCATCTCTAACTTCCTTCACTCCTTAGAGAGGGGTCAAGTCCTTCCTGCTCACACACTTCTGAACACAGTAGATGTTGAACTTATCTATGAAGGAATCAAATATGTACTTAAGGTGACTCGGCAGTCTCCCAACTCCTACGTAGTGATAATGAATGGCTCGTGTGTGGAAGTGGATGTGCATCGGCTGAGTGATGGTGGCCTGCTCTTGTCTTATGACGGCAGCAGTTACACCACATACATGAAGGAAGAGGTGGACAGTGTGCAGTGA
Peptide sequence:
MVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLFRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGIKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDSVQ
IRESfinder result
IRESfinder score
0.573227579
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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