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mmu_circAcaca_595
Basic information of circular RNA
PanCircBase ID:
mmu_circAcaca_595
mm39_Chr. Position ID:
chr11|84254489|84263540|+
Gene name:
Acaca
Transcript:
ENSMUST00000103201.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
46,47,48,49,50
Exon/intron offsets:
0,663,5007,7837,8915
Genomic length:
9051
Exon/intron sizes in nt:
14,41,21,97,97,136
Flanking exon/introns:
chr11:84237641-84254489|chr11:84263540-84271327
Splice length:
595
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_78349
Human Ortholog IDs:
Mature spliced Sequence:
GTCCTTGGTCGGGAAGTATACACCTCCAACAACCAGCTTGGGGGCATCCAGATTATGCACAACAATGGGGTTACCCACTCCACTGTTTGTGATGACTTTGAGGGAGTCTTCACAGTCTTACACTGGCTGTCATACATGCCGAAGAGCGTACACAGTTCAGTTCCTCTCCTGAATTCCAAGGATCCTATAGATAGAATCATCGAGTTTGTTCCCACAAAGGCCCCATATGATCCTCGGTGGATGCTAGCAGGCCGTCCTCACCCAACCCAGAAAGGCCAATGGTTGAGTGGGTTTTTTGACTATGGATCTTTCTCAGAAATCATGCAGCCCTGGGCACAGACCGTGGTAGTTGGCAGAGCCAGGTTAGGGGGAATACCCGTGGGAGTAGTTGCTGTAGAAACCCGAACAGTGGAACTCAGTATCCCAGCTGATCCTGCGAACCTGGATTCTGAAGCCAAGATAATCCAGCAGGCCGGCCAGGTTTGGTTCCCAGACTCTGCATTTAAGACCTATCAAGCTATCAAGGACTTTAACCGTGAAGGGCTACCTCTAATGGTCTTTGCCAACTGGAGAGGTTTCTCTGGTGGGATGAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGACTTTAACCGTGAAGGGC
58.84
GGTAACCCCATTGTTGTGCA
58.67
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGACTTTAACCGTGAAGGGCT
60.27
GGTAACCCCATTGTTGTGCA
58.67
146
PCR template sequence:
TCTGCATTTAAGACCTATCAAGCTATCAAGGACTTTAACCGTGAAGGGCTACCTCTAATGGTCTTTGCCAACTGGAGAGGTTTCTCTGGTGGGATGAAAGGTCCTTGGTCGGGAAGTATACACCTCCAACAACCAGCTTGGGGGCATCCAGATTATGCACAACAATGGGGTTACCCACTCCACTGTTTGTGATGACTTTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-205-5p(1), mmu-miR-495-3p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTGGGATGAAAGGTCCTTGGTCGGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACACA_595_ORF_1
0.7759
0.034171501
0.706740207
633
ORF sequence:
ATGCACAACAATGGGGTTACCCACTCCACTGTTTGTGATGACTTTGAGGGAGTCTTCACAGTCTTACACTGGCTGTCATACATGCCGAAGAGCGTACACAGTTCAGTTCCTCTCCTGAATTCCAAGGATCCTATAGATAGAATCATCGAGTTTGTTCCCACAAAGGCCCCATATGATCCTCGGTGGATGCTAGCAGGCCGTCCTCACCCAACCCAGAAAGGCCAATGGTTGAGTGGGTTTTTTGACTATGGATCTTTCTCAGAAATCATGCAGCCCTGGGCACAGACCGTGGTAGTTGGCAGAGCCAGGTTAGGGGGAATACCCGTGGGAGTAGTTGCTGTAGAAACCCGAACAGTGGAACTCAGTATCCCAGCTGATCCTGCGAACCTGGATTCTGAAGCCAAGATAATCCAGCAGGCCGGCCAGGTTTGGTTCCCAGACTCTGCATTTAAGACCTATCAAGCTATCAAGGACTTTAACCGTGAAGGGCTACCTCTAATGGTCTTTGCCAACTGGAGAGGTTTCTCTGGTGGGATGAAAGGTCCTTGGTCGGGAAGTATACACCTCCAACAACCAGCTTGGGGGCATCCAGATTATGCACAACAATGGGGTTACCCACTCCACTGTTTGTGA
Peptide sequence:
MHNNGVTHSTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKGPWSGSIHLQQPAWGHPDYAQQWGYPLHCL
IRESfinder result
IRESfinder score
0.643544176
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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