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mmu_circAcaca_1972
Basic information of circular RNA
PanCircBase ID:
mmu_circAcaca_1972
mm39_Chr. Position ID:
chr11|84183716|84237641|+
Gene name:
Acaca
Transcript:
ENSMUST00000103201.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
15
Exon/intron indexes:
31,32,33,34,35,36,37,38,39,40,41,42,43,44,45
Exon/intron offsets:
0,1006,2047,7177,7530,14226,18376,19836,22883,27635,29592,45423,49036,53075,53814
Genomic length:
53925
Exon/intron sizes in nt:
1,44,97,10,85,74,54,22,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:84181137-84183716|chr11:84237641-84254489
Splice length:
1972
Other database IDs:
circBase: hsa_circ_0043225, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_20323
Human Ortholog IDs:
Mature spliced Sequence:
AATGTCCTTTGCCTCCAACCTCAACCACTATGGCATGACTCATGTAGCTAGTGTCAGCGATGTTCTGTTGGACAACGCCTTCACGCCACCTTGTCAGCGGATGGGCGGAATGGTCTCTTTCCGGACCTTTGAAGATTTTGTCAGGATCTTTGATGAAATAATGGGCTGCTTCTGTGACTCCCCACCCCAAAGCCCCACATTCCCAGAGTCTGGTCATACTTCGCTCTATGATGAAGACAAGGTCCCCAGGGATGAACCAATACATATTCTGAATGTGGCTATCAAGACTGATGGCGATATTGAGGATGACAGGCTTGCAGCTATGTTCAGAGAGTTCACCCAGCAGAATAAAGCTACTTTGGTTGAGCATGGCATCCGGCGACTTACGTTCCTAGTTGCACAAAAGGATTTCAGAAAACAAGTCAACTGTGAGGTGGATCAGAGATTTCATAGAGAATTCCCCAAATTTTTCACATTCCGAGCAAGGGATAAGTTTGAGGAGGACCGCATTTATCGACACCTGGAGCCTGCTCTGGCTTTCCAGTTAGAGTTGAACCGGATGAGAAATTTTGACCTTACTGCCATCCCATGTGCTAATCACAAGATGCACCTGTACCTTGGGGCTGCTAAGGTGGAAGTAGGCACAGAAGTGACTGACTACAGGTTCTTTGTTCGTGCGATCATCAGGCACTCTGATCTGGTCACAAAGGAAGCTTCTTTCGAATATCTACAAAATGAAGGGGAACGACTGCTCCTGGAAGCTATGGATGAATTGGAAGTTGCTTTTAATAATACAAATGTCCGCACTGACTGTAACCACATCTTCCTCAACTTTGTGCCCACGGTCATCATGGACCCATCAAAGATTGAAGAATCTGTGCGGAGCATGGTAATGCGCTATGGAAGTCGGCTATGGAAATTGCGGGTCCTCCAGGCAGAACTGAAAATCAACATTCGCCTGACAACAACTGGAAAAGCAATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTACTACTTGGACATCAGCCTGTATAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCATATGGAGACAAGCAGGGACCACTGCATGGAATGTTAATTAATACTCCATATGTGACCAAAGACCTTCTTCAATCAAAGAGGTTCCAGGCACAGTCCTTAGGAACAACATATATATATGATATCCCAGAGATGTTTCGGCAGTCACTCATCAAACTCTGGGAGTCCATGTCCACCCAAGCATTTCTTCCTTCGCCTCCTTTGCCTTCCGACATCCTGACGTATACTGAACTGGTGTTGGATGATCAAGGCCAGCTGGTCCATATGAACAGACTTCCAGGAGGAAATGAGATTGGCATGGTAGCCTGGAAAATGAGCCTTAAAAGCCCTGAATATCCAGATGGCCGAGATATCATTGTCATCGGCAATGACATTACATATCGGATCGGTTCCTTTGGGCCTCAGGAGGATTTGCTGTTTCTCAGAGCTTCTGAACTTGCCAGAGCAGAAGGCATCCCACGCATCTACGTGGCAGCGAACAGTGGAGCTAGAATTGGACTTGCAGAAGAAATACGCCATATGTTCCATGTGGCCTGGGTAGATCCTGAAGATCCCTACAAGGGATACAAGTATTTATATCTGACACCCCAGGATTATAAGAGAGTCAGTGCCCTCAATTCTGTCCACTGTGAACATGTGGAGGATGAAGGGGAATCCAGGTACAAGATAACAGATATTATCGGGAAAGAAGAAGGACTTGGAGCAGAGAACCTTCGGGGTTCTGGAATGATTGCTGGGGAATCCTCATTGGCTTACGATGAGGTCATCACCATCAGCCTGGTTACATGCCGGGCCATTGGTATTGGGGCTTACCTTGTCCGGCTGGGACAAAGAACCATCCAGGTTGAGAATTCTCACTTAATTCTGACAGGAGCAGGTGCCCTCAACAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTGGGGCTTACCTTGTCCG
60.03
AGGCGTTGTCCAACAGAACA
60.11
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTGGGGCTTACCTTGTCCG
60.03
TGAAGGCGTTGTCCAACAGA
59.82
173
PCR template sequence:
GGCCATTGGTATTGGGGCTTACCTTGTCCGGCTGGGACAAAGAACCATCCAGGTTGAGAATTCTCACTTAATTCTGACAGGAGCAGGTGCCCTCAACAAAAATGTCCTTTGCCTCCAACCTCAACCACTATGGCATGACTCATGTAGCTAGTGTCAGCGATGTTCTGTTGGACAACGCCTTCACGCCACCTTGTCAGCGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(2), mmu-miR-27a-3p(2), mmu-miR-9-5p(1), mmu-miR-1a-3p(1), mmu-miR-206-3p(1), mmu-miR-221-3p(1), mmu-miR-205-5p(1), mmu-miR-18a-5p(1), mmu-miR-128-3p(1), mmu-miR-494-3p(1), mmu-miR-758-3p(1), mmu-miR-383-5p(1), mmu-miR-190b-5p(1), mmu-miR-335-3p(1), mmu-miR-490-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCCCTCAACAAAAATGTCCTTTGCC
siRNA sets
AAGGACATTTTTGTTGAGG, AGGACATTTTTGTTGAGGG, AAAGGACATTTTTGTTGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACACA_1972_ORF_1
1.1595
0.235625506
0.99999578
2022
ORF sequence:
ATGTCCTTTGCCTCCAACCTCAACCACTATGGCATGACTCATGTAGCTAGTGTCAGCGATGTTCTGTTGGACAACGCCTTCACGCCACCTTGTCAGCGGATGGGCGGAATGGTCTCTTTCCGGACCTTTGAAGATTTTGTCAGGATCTTTGATGAAATAATGGGCTGCTTCTGTGACTCCCCACCCCAAAGCCCCACATTCCCAGAGTCTGGTCATACTTCGCTCTATGATGAAGACAAGGTCCCCAGGGATGAACCAATACATATTCTGAATGTGGCTATCAAGACTGATGGCGATATTGAGGATGACAGGCTTGCAGCTATGTTCAGAGAGTTCACCCAGCAGAATAAAGCTACTTTGGTTGAGCATGGCATCCGGCGACTTACGTTCCTAGTTGCACAAAAGGATTTCAGAAAACAAGTCAACTGTGAGGTGGATCAGAGATTTCATAGAGAATTCCCCAAATTTTTCACATTCCGAGCAAGGGATAAGTTTGAGGAGGACCGCATTTATCGACACCTGGAGCCTGCTCTGGCTTTCCAGTTAGAGTTGAACCGGATGAGAAATTTTGACCTTACTGCCATCCCATGTGCTAATCACAAGATGCACCTGTACCTTGGGGCTGCTAAGGTGGAAGTAGGCACAGAAGTGACTGACTACAGGTTCTTTGTTCGTGCGATCATCAGGCACTCTGATCTGGTCACAAAGGAAGCTTCTTTCGAATATCTACAAAATGAAGGGGAACGACTGCTCCTGGAAGCTATGGATGAATTGGAAGTTGCTTTTAATAATACAAATGTCCGCACTGACTGTAACCACATCTTCCTCAACTTTGTGCCCACGGTCATCATGGACCCATCAAAGATTGAAGAATCTGTGCGGAGCATGGTAATGCGCTATGGAAGTCGGCTATGGAAATTGCGGGTCCTCCAGGCAGAACTGAAAATCAACATTCGCCTGACAACAACTGGAAAAGCAATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTACTACTTGGACATCAGCCTGTATAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCATATGGAGACAAGCAGGGACCACTGCATGGAATGTTAATTAATACTCCATATGTGACCAAAGACCTTCTTCAATCAAAGAGGTTCCAGGCACAGTCCTTAGGAACAACATATATATATGATATCCCAGAGATGTTTCGGCAGTCACTCATCAAACTCTGGGAGTCCATGTCCACCCAAGCATTTCTTCCTTCGCCTCCTTTGCCTTCCGACATCCTGACGTATACTGAACTGGTGTTGGATGATCAAGGCCAGCTGGTCCATATGAACAGACTTCCAGGAGGAAATGAGATTGGCATGGTAGCCTGGAAAATGAGCCTTAAAAGCCCTGAATATCCAGATGGCCGAGATATCATTGTCATCGGCAATGACATTACATATCGGATCGGTTCCTTTGGGCCTCAGGAGGATTTGCTGTTTCTCAGAGCTTCTGAACTTGCCAGAGCAGAAGGCATCCCACGCATCTACGTGGCAGCGAACAGTGGAGCTAGAATTGGACTTGCAGAAGAAATACGCCATATGTTCCATGTGGCCTGGGTAGATCCTGAAGATCCCTACAAGGGATACAAGTATTTATATCTGACACCCCAGGATTATAAGAGAGTCAGTGCCCTCAATTCTGTCCACTGTGAACATGTGGAGGATGAAGGGGAATCCAGGTACAAGATAACAGATATTATCGGGAAAGAAGAAGGACTTGGAGCAGAGAACCTTCGGGGTTCTGGAATGATTGCTGGGGAATCCTCATTGGCTTACGATGAGGTCATCACCATCAGCCTGGTTACATGCCGGGCCATTGGTATTGGGGCTTACCTTGTCCGGCTGGGACAAAGAACCATCCAGGTTGAGAATTCTCACTTAATTCTGACAGGAGCAGGTGCCCTCAACAAAAATGTCCTTTGCCTCCAACCTCAACCACTATGGCATGACTCATGTAGCTAG
Peptide sequence:
MSFASNLNHYGMTHVASVSDVLLDNAFTPPCQRMGGMVSFRTFEDFVRIFDEIMGCFCDSPPQSPTFPESGHTSLYDEDKVPRDEPIHILNVAIKTDGDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDILTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMSLKSPEYPDGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEVITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKNVLCLQPQPLWHDSCS
IRESfinder result
IRESfinder score
0.531366164
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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