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mmu_circAbr_379

Basic information of circular RNA
PanCircBase ID:

mmu_circAbr_379

mm39_Chr. Position ID:

chr11|76345341|76347548|-

Gene name:

Abr

Transcript:

ENSMUST00000094012.11

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

4

Exon/intron indexes:

13,12,11,10

Exon/intron offsets:

0,1093,1645,2084

Genomic length:

2207

Exon/intron sizes in nt:

7,51,05,76,123

Flanking exon/introns:

chr11:76343254-76345341|chr11:76347548-76350006

Splice length:

379

Other database IDs:

circAtlas: hsa-SCAF8_0001

Human Ortholog IDs:
Mature spliced Sequence:

AAAGCCAACAAAGGACAGAGCCGAGCCATTGAGCGCCTCAAAAAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATCCCTTTCCGGATACACAATCGGAATGGAAAGAGTTACCTGTTTCTTCTGTCTTCTGACTATGAGAGGTCGGAGTGGAGAGAAGCGATTCAGAAGCTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCTGTGGAGCTCCAGGTGCTCACGGGATCCTGTTTCAAGCTTAGGACTGTGCACAACATTCCTGTCACCAGCAATAAAGATGATGATGAGTCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCCA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CTCCAGGACTGTACGGCTTC

59.83

TTGGGGAATTGAGCAGCAGT

59.89

154

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TCTCCAGGACTGTACGGCTT

60.25

TTGGGGAATTGAGCAGCAGT

59.89

155

PCR template sequence:

ATTCCTGTCACCAGCAATAAAGATGATGATGAGTCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCCAAAAGCCAACAAAGGACAGAGCCGAGCCATTGAGCGCCTCAAAAAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATCCCTTTCC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

4

Interacting miRNAs (#binding sites)

mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-138-5p(1), mmu-miR-339-3p(1)

siRNAs for circular RNA silencing
Junction sequence

AAACAGTCAGCCAAAAGCCAACAAAG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCABR_379_ORF_1

1.0815

0.308894742

0.78649908

456

ORF sequence:

ATGATGATGAGTCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCCAAAAGCCAACAAAGGACAGAGCCGAGCCATTGAGCGCCTCAAAAAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATCCCTTTCCGGATACACAATCGGAATGGAAAGAGTTACCTGTTTCTTCTGTCTTCTGACTATGAGAGGTCGGAGTGGAGAGAAGCGATTCAGAAGCTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCTGTGGAGCTCCAGGTGCTCACGGGATCCTGTTTCAAGCTTAGGACTGTGCACAACATTCCTGTCACCAGCAATAAAGATGATGATGAGTCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCC

Peptide sequence:

MMMSLQDCTASFMSSSTLPRALNSQPKANKGQSRAIERLKKKMFENEFLLLLNSPTIPFRIHNRNGKSYLFLLSSDYERSEWREAIQKLQKKDLQAFVLSSVELQVLTGSCFKLRTVHNIPVTSNKDDDESPGLYGFLHVIVHSAKGFKQSA

IRESfinder result

IRESfinder score

0.505903466

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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