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mmu_circAbi2_431
Basic information of circular RNA
PanCircBase ID:
mmu_circAbi2_431
mm39_Chr. Position ID:
chr1|60486418|60492921|+
Gene name:
Abi2
Transcript:
ENSMUST00000052332.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,818,6344
Genomic length:
6503
Exon/intron sizes in nt:
14,71,25,159
Flanking exon/introns:
chr1:60483241-60486418|chr1:60492921-60509824
Splice length:
431
Other database IDs:
circAtlas: mmu-Abi2_0006
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAATCGAACTTACAGCAGCAGTGGGAGCAGTGGAGGAAGTCACCCAAGTAGTCGGAGCAGCAGTCGGGAGAACAGCGGAAGTGGTAGTGTGGGGGTACCCATTGCTGTGCCTACTCCGTCTCCTCCAAGCGTCTTTCCAGGTCATCCTGTTCAGTTCTACAGCATGAACAGGCCTGCCTCTCGCCATACACCACCTACAATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCGTTGCCCTATAGACGT
59.62
GCCATATTACGGGTTGGGCT
60.18
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCTATAGACGTCCTCCT
59.15
GCCATATTACGGGTTGGGCT
60.18
174
PCR template sequence:
ATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAGGCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-207(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
AACCAGAATCCAGGCGGCACTCCCCC
siRNA sets
AGTGCCGCCTGGATTCTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCABI2_431_ORF_1
1.0832
0.073495547
0.589983974
459
ORF sequence:
ATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAATCGAACTTACAGCAGCAGTGGGAGCAGTGGAGGAAGTCACCCAAGTAGTCGGAGCAGCAGTCGGGAGAACAGCGGAAGTGGTAGTGTGGGGGTACCCATTGCTGTGCCTACTCCGTCTCCTCCAAGCGTCTTTCCAGGTCATCCTGTTCAGTTCTACAGCATGAACAGGCCTGCCTCTCGCCATACACCACCTACAATAGGGGGCTCGTTGCCCTATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAACCAGAATCCAGGCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGA
Peptide sequence:
MAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPGHPVQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYNQNPGGTPPIEHWSQCVLQWYQMITYLAQPVIWLPRSRAL
IRESfinder result
IRESfinder score
0.686588397
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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