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mmu_circAamp_156
Basic information of circular RNA
PanCircBase ID:
mmu_circAamp_156
mm39_Chr. Position ID:
chr1|74323044|74323200|-
Gene name:
Aamp
Transcript:
ENSMUST00000006462.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
156
Exon/intron sizes in nt:
156
Flanking exon/introns:
chr1:74322372-74323044|chr1:74323200-74323661
Splice length:
156
Other database IDs:
circAtlas: mmu-Aamp_0012
Human Ortholog IDs:
Mature spliced Sequence:
ATGATCTGGCCCAGGAGATGGAAGACGTGGACTTTGAGGAAGAGGAAGAAGAAGAAGAGGGCAATGACGAGGGCTGGGTCTTGGAACCCCAGGAAGGGGTGGTCGGTAGCATGGAGGGCCCGGATGATAGTGAGGTCACCTTTGCATTGCACTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGTCGGTAGCATGGAGG
59.86
CCCTCGTCATTGCCCTCTTC
60.46
131
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGGTCGGTAGCATGGAG
59.86
CCCTCGTCATTGCCCTCTTC
60.46
132
PCR template sequence:
TCTTGGAACCCCAGGAAGGGGTGGTCGGTAGCATGGAGGGCCCGGATGATAGTGAGGTCACCTTTGCATTGCACTCAGATGATCTGGCCCAGGAGATGGAAGACGTGGACTTTGAGGAAGAGGAAGAAGAAGAAGAGGGCAATGACGAGGGCTGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-130a-3p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-721(1), mmu-miR-300-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCATTGCACTCAGATGATCTGGCCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAAMP_156_ORF_1
1.2591
-0.095966725
0.259268894
312
ORF sequence:
ATGATCTGGCCCAGGAGATGGAAGACGTGGACTTTGAGGAAGAGGAAGAAGAAGAAGAGGGCAATGACGAGGGCTGGGTCTTGGAACCCCAGGAAGGGGTGGTCGGTAGCATGGAGGGCCCGGATGATAGTGAGGTCACCTTTGCATTGCACTCAGATGATCTGGCCCAGGAGATGGAAGACGTGGACTTTGAGGAAGAGGAAGAAGAAGAAGAGGGCAATGACGAGGGCTGGGTCTTGGAACCCCAGGAAGGGGTGGTCGGTAGCATGGAGGGCCCGGATGATAGTGAGGTCACCTTTGCATTGCACTCAG
Peptide sequence:
MIWPRRWKTWTLRKRKKKKRAMTRAGSWNPRKGWSVAWRARMIVRSPLHCTQMIWPRRWKTWTLRKRKKKKRAMTRAGSWNPRKGWSVAWRARMIVRSPLHCTQ
IRESfinder result
IRESfinder score
0.172973486
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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