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mmu_circ4932438A13Rik_722
Basic information of circular RNA
PanCircBase ID:
mmu_circ4932438A13Rik_722
mm39_Chr. Position ID:
chr3|36949954|36974182|+
Gene name:
4932438A13Rik
Transcript:
ENSMUST00000057272.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
13,14,15,16,17,18
Exon/intron offsets:
0,10885,12326,21812,22646,24049
Genomic length:
24228
Exon/intron sizes in nt:
79,92,11,81,56,98,179
Flanking exon/introns:
chr3:36949564-36949954|chr3:36974182-36974328
Splice length:
722
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAAACAAATGCTGTGCATGTAAATGTAGGGGCTGGCTCATATTTAGAAATTAATATTCCGATGACAGTTGACGAAAATGGTTATACCCCTGCTATTAAAGGACAACTATTACATGTTGATGCTACTACCAGTATGCAATATCGGACCCTTTTAGAAGCAGAGATGTTAGCATTCCACATCAATGCCAGCTACCCCAGAATTTGGAACATGCCACAGACATGGCAATGTGAATTGGAAGTATATAAAGCCACATACCACTTCATCTTTGCACAGAAGAACTTCTTTACAGATCTAATTCAAGACTGGTCTAGTGATAATGCTCCAGACATTTTCTCATTTGTTCCATACACATGGAATTTTAAAATCATGTTTCATCAGTTTGAGATGATTTGGGCTGCCAATCAACATAACTGGATCGACTGTTCCACTAAACAACAGGAGAACGTGTATCTGGCAGCCTGTGGAGAAACACTGAACATTGACTTTTCTTTGCCTTTTACGGACTTTGTTCCAGCTACGTGCAATACCAGATTCTCCTTAAGAGGAGAAGATGTTGATCTTCATTTATTTCTACCAGATTGCCACCCTAGTAAATATTCATTATTTATGCTGGTAAAGAATTGTCACCCAAATAAGATGGTTCCTGAAACTGGTATTCCTGCCGAGTGTCAGAGTGGCCAGAAAACAGTTAAACCAAAGTGGCGCAACGTTACTCAGGAAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTCCTGCCGAGTGTCAGAG
59.47
TTCGTCAACTGTCATCGGAA
57.2
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTCCTGCCGAGTGTCAGAG
59.47
TTTCGTCAACTGTCATCGGA
57.2
144
PCR template sequence:
GTCACCCAAATAAGATGGTTCCTGAAACTGGTATTCCTGCCGAGTGTCAGAGTGGCCAGAAAACAGTTAAACCAAAGTGGCGCAACGTTACTCAGGAAAAGAAACAAATGCTGTGCATGTAAATGTAGGGGCTGGCTCATATTTAGAAATTAATATTCCGATGACAGTTGACGAAAATGGTTATACCCCTGCTATTAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-23b-3p(1), mmu-miR-132-3p(1), mmu-miR-23a-3p(1), mmu-miR-25-3p(1), mmu-miR-212-3p(1), mmu-miR-182-5p(1), mmu-miR-130b-3p(1), mmu-miR-511-3p(1), mmu-miR-3470b(1), mmu-miR-338-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACTCAGGAAAAGAAACAAATGCTG
siRNA sets
ATTTGTTTCTTTTCCTGAG, TTTGTTTCTTTTCCTGAGT, CAGCATTTGTTTCTTTTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRC4932438A13RIK_722_ORF_1
0.7572
-0.01401651
0.733002208
684
ORF sequence:
ATGACAGTTGACGAAAATGGTTATACCCCTGCTATTAAAGGACAACTATTACATGTTGATGCTACTACCAGTATGCAATATCGGACCCTTTTAGAAGCAGAGATGTTAGCATTCCACATCAATGCCAGCTACCCCAGAATTTGGAACATGCCACAGACATGGCAATGTGAATTGGAAGTATATAAAGCCACATACCACTTCATCTTTGCACAGAAGAACTTCTTTACAGATCTAATTCAAGACTGGTCTAGTGATAATGCTCCAGACATTTTCTCATTTGTTCCATACACATGGAATTTTAAAATCATGTTTCATCAGTTTGAGATGATTTGGGCTGCCAATCAACATAACTGGATCGACTGTTCCACTAAACAACAGGAGAACGTGTATCTGGCAGCCTGTGGAGAAACACTGAACATTGACTTTTCTTTGCCTTTTACGGACTTTGTTCCAGCTACGTGCAATACCAGATTCTCCTTAAGAGGAGAAGATGTTGATCTTCATTTATTTCTACCAGATTGCCACCCTAGTAAATATTCATTATTTATGCTGGTAAAGAATTGTCACCCAAATAAGATGGTTCCTGAAACTGGTATTCCTGCCGAGTGTCAGAGTGGCCAGAAAACAGTTAAACCAAAGTGGCGCAACGTTACTCAGGAAAAGAAACAAATGCTGTGCATGTAA
Peptide sequence:
MTVDENGYTPAIKGQLLHVDATTSMQYRTLLEAEMLAFHINASYPRIWNMPQTWQCELEVYKATYHFIFAQKNFFTDLIQDWSSDNAPDIFSFVPYTWNFKIMFHQFEMIWAANQHNWIDCSTKQQENVYLAACGETLNIDFSLPFTDFVPATCNTRFSLRGEDVDLHLFLPDCHPSKYSLFMLVKNCHPNKMVPETGIPAECQSGQKTVKPKWRNVTQEKKQMLCM
IRESfinder result
IRESfinder score
0.747022803
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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