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mmu_circ4932438A13Rik_210
Basic information of circular RNA
PanCircBase ID:
mmu_circ4932438A13Rik_210
mm39_Chr. Position ID:
chr3|36960839|36962398|+
Gene name:
4932438A13Rik
Transcript:
ENSMUST00000057272.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
14,15
Exon/intron offsets:
0,1441
Genomic length:
1559
Exon/intron sizes in nt:
92,118
Flanking exon/introns:
chr3:36950033-36960839|chr3:36962398-36971766
Splice length:
210
Other database IDs:
circBase: mmu_circ_0001119, CIRCpedia: MMU_CIRCpedia_225679, circAtlas: mmu-4932438A13Rik_0123
Human Ortholog IDs:
Mature spliced Sequence:
GTTATACCCCTGCTATTAAAGGACAACTATTACATGTTGATGCTACTACCAGTATGCAATATCGGACCCTTTTAGAAGCAGAGATGTTAGCATTCCACATCAATGCCAGCTACCCCAGAATTTGGAACATGCCACAGACATGGCAATGTGAATTGGAAGTATATAAAGCCACATACCACTTCATCTTTGCACAGAAGAACTTCTTTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGGAACATGCCACAGACA
57.94
GGGTCCGATATTGCATACTGGT
59.96
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGGAACATGCCACAGACA
57.94
CTCTGCTTCTAAAAGGGTCCGA
59.77
173
PCR template sequence:
TACCCCAGAATTTGGAACATGCCACAGACATGGCAATGTGAATTGGAAGTATATAAAGCCACATACCACTTCATCTTTGCACAGAAGAACTTCTTTACAGGTTATACCCCTGCTATTAAAGGACAACTATTACATGTTGATGCTACTACCAGTATGCAATATCGGACCCTTTTAGAAGCAGAGATGTTAGCATTCCACAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-23a-3p(1), mmu-miR-130b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AACTTCTTTACAGGTTATACCCCTGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRC4932438A13RIK_210_ORF_1
0.6535
0.036849929
0.223420076
366
ORF sequence:
ATGCAATATCGGACCCTTTTAGAAGCAGAGATGTTAGCATTCCACATCAATGCCAGCTACCCCAGAATTTGGAACATGCCACAGACATGGCAATGTGAATTGGAAGTATATAAAGCCACATACCACTTCATCTTTGCACAGAAGAACTTCTTTACAGGTTATACCCCTGCTATTAAAGGACAACTATTACATGTTGATGCTACTACCAGTATGCAATATCGGACCCTTTTAGAAGCAGAGATGTTAGCATTCCACATCAATGCCAGCTACCCCAGAATTTGGAACATGCCACAGACATGGCAATGTGAATTGGAAGTATATAAAGCCACATACCACTTCATCTTTGCACAGAAGAACTTCTTTACA
Peptide sequence:
MQYRTLLEAEMLAFHINASYPRIWNMPQTWQCELEVYKATYHFIFAQKNFFTGYTPAIKGQLLHVDATTSMQYRTLLEAEMLAFHINASYPRIWNMPQTWQCELEVYKATYHFIFAQKNFFT
IRESfinder result
IRESfinder score
0.487144652
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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