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mmu_ciPrkar1a_657
Basic information of circular RNA
PanCircBase ID:
mmu_ciPrkar1a_657
mm39_Chr. Position ID:
chr11|109556812|109557469|+
Gene name:
Prkar1a
Transcript:
ENSMUST00000106677.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
10
Exon/intron offsets:
0
Genomic length:
657
Exon/intron sizes in nt:
657
Flanking exon/introns:
chr11:109556812-109557469
Splice length:
657
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGAGAGCTCTGCGAGTGACTCCTTACCATGCGTCACCTAAAGGAGGGGCAGCCAGTTCTGATTTGTAGCTTTAGTTTTATTTAGTCGTTATCTTTTACTTCAGAATACAGCTTGCTGTGTATAGTCTGCTACAGTTTTAAAGAATTAGAGTCAGAATCCTGCAAGGGCCCTAGACATGATTGGTGAGATGGCTCTCTACTACACTAAAAAGCCCCTCAGCCATAACAGATACGGTGATTCTGAGCCAAAGTGCTTAGGCTAGTTAGTAATGATTGACAATTCTAAAAGTCACTGTTACTGAAACATCTCCCTATCATCCTGCTTGCCAAGCAGGTCCAGATGGCAGCTTCAGAGTCTGAACTCTTGTCGCACTGCAGATGCAGCCATGGAGATGTGAGCCCTGAGTGTGATAGTCTTTAAAATCTTATTTCCAAAGATGCAGCAGGCTTGAGCTTGACCCTTTACGTAAACTTTACTCACCGGAGTCTGAACTCCTTTGTAAGTGCCTGAGCTTGATACGAGCCTTGATAGCGGGGTGGATGCTGGTTCAGTGGAAGTCAGCGTTGAAATAACTGCTCAGTTCACTCAGGAACCGGCGATTGCACAGTATGTGTTAGGACCGTGTGTTTAGCTTCTTGCGTTGGTGTGATTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGTTCACTCAGGAACCGG
59.32
CAAATCAGAACTGGCTGCCC
59.47
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGTTCACTCAGGAACCGG
59.32
AAATCAGAACTGGCTGCCCC
60.61
145
PCR template sequence:
AGTCAGCGTTGAAATAACTGCTCAGTTCACTCAGGAACCGGCGATTGCACAGTATGTGTTAGGACCGTGTGTTTAGCTTCTTGCGTTGGTGTGATTACAGGTAGAGAGCTCTGCGAGTGACTCCTTACCATGCGTCACCTAAAGGAGGGGCAGCCAGTTCTGATTTGTAGCTTTAGTTTTATTTAGTCGTTATCTTTTAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-434-3p(1), mmu-miR-22-3p(1), mmu-miR-127-5p(1), mmu-miR-802-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTGTGATTACAGGTAGAGAGCTCTG
siRNA sets
CTCTACCTGTAATCACACC, TCTCTACCTGTAATCACAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.775255468
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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