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mmu_ciPrepl_2545
Basic information of circular RNA
PanCircBase ID:
mmu_ciPrepl_2545
mm39_Chr. Position ID:
chr17|85373767|85376312|-
Gene name:
Prepl
Transcript:
ENSMUST00000072406.5
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
10
Exon/intron offsets:
0
Genomic length:
2545
Exon/intron sizes in nt:
2545
Flanking exon/introns:
chr17:85373725-85376312
Splice length:
2545
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTACCACCCTGTGCCACTGCTCAGAGAGAAAATGGTGGCCTTTAAGTTTAAACAGAGCGGTGGATTTATCTTAATCCCATTATTTATTCTCCCCTGACAACCTTTATAGATTCTGCAGGATTGCCATTGAGAGACTAATGGCTGTCCCTGTGACCCTTTTCCGTTTGTCAGGACATTTTGAAATGCATCTATGTACCAGGATTCCAGAAGCCTGGTGGATTGTCCTGGGGGTCTGTGTGCACTGCCCTTGTCCATGTGACAGCATCCTGCCCAGCTGCATCTTATTCTGGACATAGGGAATAGGTCTCTCTTTTCAGACACTCTGTATGTTGTTTTATGTTTTGCTGTATAGCAGGTCAGTCTGGCTTCATACTCACAGCCTCCGACCCAAGCACCAGGACTTAAGTATGTACCACCATGTCTGCTACAGAGACTCTATTCTCCATTTCTTTCTGGTCCTGTCCTTTGATGAGCTCTCAGTTCCGGAAATGCCCTACAGTCTTTTCCTATTCTCCAAAACTGTACAGTGTCTGGCTTCATATTCATCTTTTCCTATCTCCTACCTAGATACTCTGGAAAATAATTACTGGGGGATTCAGGGTAGCTCTTTTCAGGGTGTGTGTGTGTGCACACGGGTGTGTGCATGTTTGGAGCAAGTATTAGTAAGTTAAATATTAAATAACTTGATCTAGAAATCTTTTTTTAATATTCTTTATAGAGGAAATAAACCTAGCCACAAAGATAATAACAAAATCACTTACAATATATAATATGCAGCTAACAAATTTTAGAAATTAGAAATAACCAGAGCCGAGAAGTGGTGGTGCACCCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTAAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCAAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAAACAAAAAAGAAAAGAAATAACCAGAAGTGCATGTGTAAGGACATTTAATTCAGCTGCTCATTACAGTGAAGAATTAGGGACAGCCTGCATGTTCAACACAAAATGTTCATGTATGAGTCAAAGTCATTGATATAAGCACCCAGAGTTATGGCTAACTGAAGCAGGGAGGGTTTACTGGAGTATTAGGTAAGAGTGGGTGTGTGGAGAACCAGTGCCAGAGGCTGCCCAGCCAGGAACTGCCCAGCGGGGCTGCCACTGGTGAGCACAGCACCTCTCACTGCCATTGCTGCCCCTGAAAACTGGGGGCACACACACTTGCCTGAATTAGGTTCCCTGGGTCACCCTCTGTGCAGTACAAGGGCCCTCTTTACAAAGCTGGCAGCTTTGCACATCAGCAAAGCCATCCTGTCAGGCAGCCAGGATTACAGCTAGACTCAGTAAATGGTTCTGTGCAGGACAACGCTGGAGAGTCCACGCGCTGGAAGCCGGCGTCCCTTTAGCTAAATGTCTTAGCTTAGTTTTACTTGAGGTGTGTGGGTGTTTTTCCTGTGTGTGTGCATGTGTGTCACGCACATTCCTTTCTTCTTGGCCATTTTCTAGATCTTACCAAGTAGGATTATGAGTTTTAGAGTAACAGTGCAGCAGGTGAGGCAGAGTTTGTGTGCTGTTGATGCTTTAAAAGTTCTCACTTTTAGAGTTGTCTAGTCACAGGAAAGGAACAAAATCTATCCTAACTATTTTTAGTGTATGGCTCGATAATGTTAAACATTTACTCTATTATGCAGTCAGTCTCCAAATCACAATTTTTATCTTTTCAAAACTGAAACACATGTAGCACTGAACGCTCTCCATTCCCTCCCTCCCAAGCCGTGGGAAACCTCCTCCTACACTGTGTTCAAAGTCACCTCATGTGATGTCATGCACAGTCGCCTCATGTGATGTCATGCACATGTGATGTCATGCACAATCAGTCACCTCATGTGGTGTCATGCACAATCAGTCACCTCATGTGATGTCATGCACACTCAGTCACCTCATGTGGTGTCATGCCAAGTTAATCCTTGATGCTGCCTCTCCCACTTAGCTGACACTGACACCATCCATTGGGAGAGGCCCAGACTCTGGTCTGTGTGCACACAGCAGTTTGCTTCTTCCCTGTCCTAGTGTTGCTTTCAGACCCCGTCTCTAGCTCTTTGGGGCACATCTCTAGGAAGGGGTTCTAGATCTAAAGGTGAACGGCACCTTCATTTTTAAGTGACAGAGATTTACACTCTAGCCAATAGTGTGAAAATAGTGCAGCTTCTCTCCATCATCACCAACTGGACATGTTCTGATTATTGGGGGGGGGGTGTTTTTAATATAGTCGCCATCCTAATGAATGCAGAGCTGTTATTTGTAAAAATAGAAGAAGAGGCAAACAAAAATTGTGAAATTACAAGGTAAATTTTCTTGTTATATACATGTCTGTAAGGGGTGTGAATGCTCTTTAGCCATCTGTGAATTACCAGCTGTTTTAAGCCTGAGCCGTCTTCTTTGTCACCTGGATGTGTGTTTATGCAGGAAGCTGTAGTGATGCTCATTATACCAACCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTGTTTTAAGCCTGAGCCG
59.83
TCCACCGCTCTGTTTAAACT
57.08
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCTGAGCCGTCTTCTTTG
60.32
TCCACCGCTCTGTTTAAACT
57.08
143
PCR template sequence:
CATCTGTGAATTACCAGCTGTTTTAAGCCTGAGCCGTCTTCTTTGTCACCTGGATGTGTGTTTATGCAGGAAGCTGTAGTGATGCTCATTATACCAACCAGTGAGTACCACCCTGTGCCACTGCTCAGAGAGAAAATGGTGGCCTTTAAGTTTAAACAGAGCGGTGGATTTATCTTAATCCCATTATTTATTCTCCCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
30
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(3), mmu-miR-34c-5p(2), mmu-miR-34b-5p(2), mmu-miR-344-3p(2), mmu-miR-210-3p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-221-3p(1), mmu-miR-26a-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-18a-5p(1), mmu-miR-328-3p(1), mmu-miR-217-5p(1), mmu-miR-709(1), mmu-miR-320-3p(1), mmu-miR-26b-5p(1), mmu-miR-22-3p(1), mmu-miR-370-3p(1), mmu-miR-340-5p(1), mmu-miR-384-3p(1), mmu-miR-466a-5p(1), mmu-miR-466p-5p(1), mmu-miR-410-3p(1), mmu-miR-5101(1), mmu-miR-3087-3p(1), mmu-miR-3470b(1), mmu-miR-574-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTATACCAACCAGTGAGTACCACCC
siRNA sets
GTGGTACTCACTGGTTGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.78506014
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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