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mmu_ciPpm1g_283
Basic information of circular RNA
PanCircBase ID:
mmu_ciPpm1g_283
mm39_Chr. Position ID:
chr5|31362058|31362341|-
Gene name:
Ppm1g
Transcript:
ENSMUST00000202294.4
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
283
Exon/intron sizes in nt:
283
Flanking exon/introns:
chr5:31362031-31362341
Splice length:
283
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTGTGGGGGAGGGAATGAGTGACTTTGGAAAGGACAAGGGACTGGTATAAGTCTCGTGAGTTTTGATATTTGGAAGTATATCCCCTTGGTGTTTTAGTCTTTAATTCATCATCAGGAGAATTAATTCTACAGTAGGAACTCTTAAGGGTAGTCTGAATAGGTTTGCCTTGGAAAGGGTACAGGAGCATGCTGCTGTCTCTCTGGACAGCTCTTCCTATTGATTGTTAAAGCTCTCATATAGATAGTTGGGTCAGAGGGGTGCACTCCTACCGAGGAGCTTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATGCTGCTGTCTCTCTGGA
59.46
CCAGTCCCTTGTCCTTTCCA
59.23
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGCTGCTGTCTCTCTGGAC
59.46
CCAGTCCCTTGTCCTTTCCA
59.23
142
PCR template sequence:
GGAGCATGCTGCTGTCTCTCTGGACAGCTCTTCCTATTGATTGTTAAAGCTCTCATATAGATAGTTGGGTCAGAGGGGTGCACTCCTACCGAGGAGCTTCGTGTGTGGGGGAGGGAATGAGTGACTTTGGAAAGGACAAGGGACTGGTATAAGTCTCGTGAGTTTTGATATTTGGAAGTATATCCCCTTGGTGTTTTAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-322-5p(1), mmu-miR-185-5p(1), mmu-miR-224-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACCGAGGAGCTTCGTGTGTGGGGGAG
siRNA sets
ACACACGAAGCTCCTCGGT, CTCCCCCACACACGAAGCT, CACACACGAAGCTCCTCGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.859143053
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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