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mmu_ciPpil4_2221
Basic information of circular RNA
PanCircBase ID:
mmu_ciPpil4_2221
mm39_Chr. Position ID:
chr10|7680786|7683007|+
Gene name:
Ppil4
Transcript:
ENSMUST00000015901.11
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
9
Exon/intron offsets:
0
Genomic length:
2221
Exon/intron sizes in nt:
2221
Flanking exon/introns:
chr10:7680786-7683034
Splice length:
2221
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTCATAATGTAAATCTACCAGATCTGGTTTTTCTTACTTACTTTTTGCTTTGGCGAATGCTTCCATGTGTCTGAGATTACTGGTGTTCACCACTGCACCAGCCTGTCTGCCTCCGAGTAGTCTTAAAGATGAAAAGTTGTGCTTTCTTCAGTCTCTAGGGTGTGCTTTTCCTCAGGCATCCAAGAACATTAACTATTTTAACTTTTCATTAGAAATACATTCAACAGCTACTGATGTTAATGTTTGTTTACTATAAGTTCAGCTATAATCTGTCTGAATGTAATTGTTTTAGAAAAAGTCTTTCACATACTGTTAATTATATAACTGAGTAGCAGAAAGAGAAATGGTGGAGTGTTAAGATCTAGGCTCAATTACTGGCCAGCTACTGGAAGCGCTCACGTATTTAACTCTTCTTCCCAAGGTTTTTCCTCCTTGGTGTGCAGTGAGAGGACTGGATCAGGTAGCTGCTGCTAGATCTGCCTTAGTGAGGATAGGTCGGTTGGCAAGTGCTTGCTTCAAGTCAGACATTACCTCATTTTTCTGTGTGTAGTATGTACAGCTCTTAGTTCTCCATTATGCACTGAGCTCCAGAAGCTCAGCGGTAGACTTCGACCTTAATTAGCAGCGTTTCCCACGGTGCACAGACATGCATTCAGGGTAACACTCACCCATATAAAAATTTAAAATTTCAGCCGGGCGGTGGTGGCGCATGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAATGAAGAAATAAATAAAATAAAATAAAATCTCATAATTCAAGCTTAATAATATTGTTATTAATATGTGGGGGGTACACACATACGGCAGCCCACATGTGGAGGTCAGAGGACAACTTTTGGGAGTTGATTCTTCCATCTTATTCTAAGGCAAGATTTCTTGTGTTTCTGTTTCTGCTGTGCTGCAGAGCTAGCTGACCCTAGGCCTCCAAGAGTCTTCTGTTTCTACCTCCCATCTTGCTGTGGGAGCGCTGTGAATATAGATGAGCCATCATGTCTGGCTTCTTCTTTAGTTTGAGGGATAAAACCCAGGTTGTTAGGCACGTGTGGCAAGCACAGCAGCCAGCTGAGTCACCTCACTGGCCCAGATGTGCTCCTGAAATGAAGTACCCATGCAAGATATCTTGCTCCCTCACTCTGCCTTTGTGCCGTCAAGTGTTAGATGGTTACTTGCCTGCTTGCCTCCTTACTGCCCTGCCTTTTATTGACTTTTAGGAACTCTATATGTTACGAATGTAAGCTCTTTTTAAAAATATACGTCCTATGTATCTCTCTTCTGTTCTGTGGATTACCCATTTGATAAACACAAAAGTAATAGTTTTAATTTAGTTTCATTTGTCAACATTTTCTCAAGATGGCTTCTAAAAATCTGTTTACCTAAAGACATGAGAATATTCTCTATGTTCTTTATTTATTTTAATGTAAATACATGAGTCTTTTGTCTACATATGTGCATGTGCACCGTGTATGGTGCCCTTGCCGGCCTAAGAGAGCATACTCTACATTCACTGAAATTGGAGTTAAGGGTGGTTGTGGGCTAATGGGTGCTAGAAACCAAACGTGGGTTCTCTGCAAAAGCAGGAAATGCAGGAACCTAAGCCACCTGTCTCTGCAGCCCCTCTGCACTGTCTTCATAAACCACGTTATTAGATTGTTTCCATTGGGGTCAGTGCTTTGTCTGTGTCCAATGTGGATTATGTTGCATTTCATTTGTCTGACTTTGGATACCCAGCCCCACTGAAAATAAAGGTATTCCTGTTCCCCAGAGCTCTGTGGGACCATCTTTGTTATAAATCAAGCATTCATATGTGTTTAAAGTCTAGTTCAAGGCACTGGGACCATGCTCATGTTAGACATCTCAGAGTGTGAGTCCTCTAACTTTTTTTTTTTTCATTTTTGTTTTGTTTTGTTGTTTTGAGATAGTGAAGATTATGACTAGATGGAGCCTCAAAAGTTTATTACCAGGGTTAATTGAGATAGTAGATGCAATACATTTAATATCTGGTATATAAGAAGTATTTGAATATCAGTTTTAGTAGTAATAGCCTTTTTGTATTCATATTATTTGATCTAGCTGCAGAAATCTCTCCTGAAACATTCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGTTTTAGTAGTAATAGCCTTTTTGT
56.38
GAAGCATTCGCCAAAGCAAA
58.22
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGTTTTAGTAGTAATAGCCTTTTTG
56.17
GAAGCATTCGCCAAAGCAAA
58.22
142
PCR template sequence:
CTGGTATATAAGAAGTATTTGAATATCAGTTTTAGTAGTAATAGCCTTTTTGTATTCATATTATTTGATCTAGCTGCAGAAATCTCTCCTGAAACATTCAGTATGTCATAATGTAAATCTACCAGATCTGGTTTTTCTTACTTACTTTTTGCTTTGGCGAATGCTTCCATGTGTCTGAGATTACTGGTGTTCACCACTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
31
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-181a-5p(1), mmu-let-7b-5p(1), mmu-miR-150-5p(1), mmu-miR-30e-5p(1), mmu-miR-31-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-138-5p(1), mmu-miR-495-3p(1), mmu-miR-214-3p(1), mmu-miR-30b-5p(1), mmu-miR-411-3p(1), mmu-miR-511-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-410-3p(1), mmu-miR-335-3p(1), mmu-miR-3087-3p(1), mmu-miR-188-3p(1), mmu-miR-3470b(1), mmu-miR-466b-3p(1), mmu-miR-670-5p(1), mmu-miR-1904(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGAAACATTCAGTATGTCATAATG
siRNA sets
CATTATGACATACTGAATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.865388014
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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