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mmu_ciP4hb_432
Basic information of circular RNA
PanCircBase ID:
mmu_ciP4hb_432
mm39_Chr. Position ID:
chr11|120451462|120451894|-
Gene name:
P4hb
Transcript:
ENSMUST00000166620.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
432
Exon/intron sizes in nt:
432
Flanking exon/introns:
chr11:120451376-120451894
Splice length:
432
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGAATCTTTCTGAAGCCACACTCATCTGACACACGTACACTTAGACCTGTCTCTTCTTTTGCTTTTCAATTTTGGAAAGGGATCTCTGTCCAGGTCAGCCCCTCTTGAAGGGCTACCTTTTGTTTTAATTGGTGGTGTACTTTTTTGTATGTGGAATTTGTCCCATGTGCTTGCTAAAATGTTTGGGGATCTCCTCACACTGGTAATGTCTTTCCTGTTAGAGAGGTTTATGCTACCACTTCGCATTTCATCTGTGAGGCATTTCATCTTTTAGACATGTCTTCATGTCCAGCTACTTGTTTCACCATGGCAGACCTCCCTGAGCCTTCTTCCTGCCCTGGGTAGGAAGTGTGGCTCTGGGTCTGTGGTCACTCTCCACCTTGTCCTAGTGTTGCCATGGTAGCATGGCTTTGTAGTTTGCATTTAACCTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGGGTCTGTGGTCACTCT
59.81
GCTGACCTGGACAGAGATCC
59.54
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGGGTCTGTGGTCACTCT
59.81
GGCTGACCTGGACAGAGATC
59.54
177
PCR template sequence:
CTGCCCTGGGTAGGAAGTGTGGCTCTGGGTCTGTGGTCACTCTCCACCTTGTCCTAGTGTTGCCATGGTAGCATGGCTTTGTAGTTTGCATTTAACCTGGGGAATCTTTCTGAAGCCACACTCATCTGACACACGTACACTTAGACCTGTCTCTTCTTTTGCTTTTCAATTTTGGAAAGGGATCTCTGTCCAGGTCAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-1187(1)
siRNAs for circular RNA silencing
Junction sequence
GCATTTAACCTGGGGAATCTTTCTGA
siRNA sets
TCAGAAAGATTCCCCAGGT, AAAGATTCCCCAGGTTAAA, GATTCCCCAGGTTAAATGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIP4HB_432_ORF_1
0.5077
-0.37201642
0.008154088
144
ORF sequence:
ATGGTAGCATGGCTTTGTAGTTTGCATTTAACCTGGGGAATCTTTCTGAAGCCACACTCATCTGACACACGTACACTTAGACCTGTCTCTTCTTTTGCTTTTCAATTTTGGAAAGGGATCTCTGTCCAGGTCAGCCCCTCTTGA
Peptide sequence:
MVAWLCSLHLTWGIFLKPHSSDTRTLRPVSSFAFQFWKGISVQVSPS
IRESfinder result
IRESfinder score
0.885743294
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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