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mmu_ciP4hb_240
Basic information of circular RNA
PanCircBase ID:
mmu_ciP4hb_240
mm39_Chr. Position ID:
chr11|120451654|120451894|-
Gene name:
P4hb
Transcript:
ENSMUST00000166620.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
240
Exon/intron sizes in nt:
240
Flanking exon/introns:
chr11:120451376-120451894
Splice length:
240
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGAATCTTTCTGAAGCCACACTCATCTGACACACGTACACTTAGACCTGTCTCTTCTTTTGCTTTTCAATTTTGGAAAGGGATCTCTGTCCAGGTCAGCCCCTCTTGAAGGGCTACCTTTTGTTTTAATTGGTGGTGTACTTTTTTGTATGTGGAATTTGTCCCATGTGCTTGCTAAAATGTTTGGGGATCTCCTCACACTGGTAATGTCTTTCCTGTTAGAGAGGTTTATGCTACCACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGGATCTCCTCACACTGG
60.62
GCTGACCTGGACAGAGATCC
59.54
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGGATCTCCTCACACTGG
60.62
GGCTGACCTGGACAGAGATC
59.54
157
PCR template sequence:
TTTTTTGTATGTGGAATTTGTCCCATGTGCTTGCTAAAATGTTTGGGGATCTCCTCACACTGGTAATGTCTTTCCTGTTAGAGAGGTTTATGCTACCACTGGAATCTTTCTGAAGCCACACTCATCTGACACACGTACACTTAGACCTGTCTCTTCTTTTGCTTTTCAATTTTGGAAAGGGATCTCTGTCCAGGTCAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-1187(1)
siRNAs for circular RNA silencing
Junction sequence
TTATGCTACCACTGGAATCTTTCTGA
siRNA sets
GAAAGATTCCAGTGGTAGC, AAAGATTCCAGTGGTAGCA, TCAGAAAGATTCCAGTGGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIP4HB_240_ORF_1
0.9949
-0.219731654
0.028063154
105
ORF sequence:
ATGTGGAATTTGTCCCATGTGCTTGCTAAAATGTTTGGGGATCTCCTCACACTGGTAATGTCTTTCCTGTTAGAGAGGTTTATGCTACCACTGGAATCTTTCTGA
Peptide sequence:
MWNLSHVLAKMFGDLLTLVMSFLLERFMLPLESF
IRESfinder result
IRESfinder score
0.914763804
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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