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mmu_ciNop14_294
Basic information of circular RNA
PanCircBase ID:
mmu_ciNop14_294
mm39_Chr. Position ID:
chr5|34798372|34798666|-
Gene name:
Nop14
Transcript:
ENSMUST00000041364.13
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
294
Exon/intron sizes in nt:
294
Flanking exon/introns:
chr5:34798361-34798666
Splice length:
294
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTGTGACACCCCAGGGACATCAAGAATAAATGTCCTAACATGTGCAGCATTGTTTTCAAAGTATACTTATTTGATTGGGAACTGAAACTGAGTCCGCATTTTAAAGTATTGTTGCATGTCTTGTGCTTCTTTCTGCTCTGGGTAAACTATTTCCTAGACTGCCATAATGAGCCATTTAGCCTTTCTGCTGATGTAATTCCACTTAGATTTTCTGAGCTGTGGGTGAGACATTTCATTGGCTTATGAGTAACTACTGAAATAAGGTGAGCTGAGTCCCTTTCATTGTCCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGAGCTGTGGGTGAGACA
60.47
CGGACTCAGTTTCAGTTCCCA
59.93
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGAGCTGTGGGTGAGACAT
59.39
CGGACTCAGTTTCAGTTCCCA
59.93
179
PCR template sequence:
ATGTAATTCCACTTAGATTTTCTGAGCTGTGGGTGAGACATTTCATTGGCTTATGAGTAACTACTGAAATAAGGTGAGCTGAGTCCCTTTCATTGTCCTGGTATGTGTGACACCCCAGGGACATCAAGAATAAATGTCCTAACATGTGCAGCATTGTTTTCAAAGTATACTTATTTGATTGGGAACTGAAACTGAGTCCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-203-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-690(1)
siRNAs for circular RNA silencing
Junction sequence
TTTCATTGTCCTGGTATGTGTGACAC
siRNA sets
ACACATACCAGGACAATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.832640678
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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