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mmu_ciNhlrc2_585
Basic information of circular RNA
PanCircBase ID:
mmu_ciNhlrc2_585
mm39_Chr. Position ID:
chr19|56580239|56580824|+
Gene name:
Nhlrc2
Transcript:
ENSMUST00000071423.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
585
Exon/intron sizes in nt:
585
Flanking exon/introns:
chr19:56580239-56580824
Splice length:
585
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAAGAACCTGTCTGTGCGCACATCCCTGAGTGAGCCATCCATGGAGTGCAGGGGTTCATTTCTGGCCTGTCTGTAATAACATGCAGTCAAAGCAGAGAGAGTGTGGCTATAAATACAGAGCTGGGCAGTGGTGGCGCATGACTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTCCGAGGCCAGCCTGGTCTACAGAGAGAGTTCCAGGACAGCCAGGGCTACACAAAAGAAATACAAGTAAAGCCCTCCCTCTGAGAAAGGCGCCCACCTGACTACATACAAGCATTCAGGGGACGGGAAGAGAGTGATTGCACCGGGTTTGTGGCAGTGGGTTAGCAGGAGAACTGCACTAGATGTGAGCCTACTGTGGGCAAGACAGCATGGCAGTTAGTAGAGTGCAGAGTGAGCCTACTGTGTTCCCCATTCTCTATCGCACGCTGGAACTACTGGGAAAAGTCAGAGCTTCCCAGCTTTAGAAACACTGGAATCTTAGTTTTCACTTTACCTCCTAACAAAGCACTGAAAATTTTAATAACTGCTGTTTCTTTCTCTTTAAAAAAAAATAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTCCTAACAAAGCACTGA
56.38
AGACAGGCCAGAAATGAACCC
60.27
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACTTTACCTCCTAACAAAGCACTG
60.05
AGGCCAGAAATGAACCCCTG
59.96
142
PCR template sequence:
GCTTTAGAAACACTGGAATCTTAGTTTTCACTTTACCTCCTAACAAAGCACTGAAAATTTTAATAACTGCTGTTTCTTTCTCTTTAAAAAAAAATAAAAGGTAAAGAACCTGTCTGTGCGCACATCCCTGAGTGAGCCATCCATGGAGTGCAGGGGTTCATTTCTGGCCTGTCTGTAATAACATGCAGTCAAAGCAGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-324-3p(2), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-320-3p(1), mmu-miR-495-3p(1), mmu-miR-127-5p(1), mmu-miR-297a-5p(1), mmu-miR-3470b(1), mmu-miR-670-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAAAAATAAAAGGTAAAGAACCTGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.765927776
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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