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mmu_ciMyh10_337
Basic information of circular RNA
PanCircBase ID:
mmu_ciMyh10_337
mm39_Chr. Position ID:
chr11|68692555|68692892|+
Gene name:
Myh10
Transcript:
ENSMUST00000102611.10
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
30
Exon/intron offsets:
0
Genomic length:
337
Exon/intron sizes in nt:
337
Flanking exon/introns:
chr11:68692555-68692933
Splice length:
337
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTGTGTCATCTGGGGAGCAGCGGTGCCCTAGATGTATGCAGGGACAGTGCAGCCTGGCCACAGGGACTCTGTCTCTGCATGTGGGACAGTTAATTGGGTTGAACTAAATTAAGCTCGTTACATGGTTTTTCTTCCTTTGACCCAGTCAAACACTGATGTCCTCTTTATAACTTCACCACCTTACAGACAGAGCGTGGCTTCATGGGATGCCAATACAGCTGCCATTTTACAGCTTAGCTAAGGGTTCCTTCCACTCCAGGGCTTGTGTTAAAATCATCCCATGCTGGTTTATAATATTGGAAACAAGTGTTTATTCTTGAAATGCAAAAGTCAGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTCCACTCCAGGGCTTGTG
60.61
CTGCACTGTCCCTGCATACA
60.04
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCACTCCAGGGCTTGTGTTA
59.6
TGTCCCACATGCAGAGACAG
59.68
175
PCR template sequence:
AGCTAAGGGTTCCTTCCACTCCAGGGCTTGTGTTAAAATCATCCCATGCTGGTTTATAATATTGGAAACAAGTGTTTATTCTTGAAATGCAAAAGTCAGAGTGTGTGTCATCTGGGGAGCAGCGGTGCCCTAGATGTATGCAGGGACAGTGCAGCCTGGCCACAGGGACTCTGTCTCTGCATGTGGGACAGTTAATTGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(1), mmu-miR-188-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCAAAAGTCAGAGTGTGTGTCATCT
siRNA sets
AGATGACACACACTCTGAC, ACACACTCTGACTTTTGCA, GATGACACACACTCTGACT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIMYH10_337_ORF_1
0.6219
-0.203898856
0.020763446
150
ORF sequence:
ATGCTGGTTTATAATATTGGAAACAAGTGTTTATTCTTGAAATGCAAAAGTCAGAGTGTGTGTCATCTGGGGAGCAGCGGTGCCCTAGATGTATGCAGGGACAGTGCAGCCTGGCCACAGGGACTCTGTCTCTGCATGTGGGACAGTTAA
Peptide sequence:
MLVYNIGNKCLFLKCKSQSVCHLGSSGALDVCRDSAAWPQGLCLCMWDS
IRESfinder result
IRESfinder score
0.819290207
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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