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mmu_ciMorc3_2747
Basic information of circular RNA
PanCircBase ID:
mmu_ciMorc3_2747
mm39_Chr. Position ID:
chr16|93638331|93641078|+
Gene name:
Morc3
Transcript:
ENSMUST00000044068.10
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
2747
Exon/intron sizes in nt:
2747
Flanking exon/introns:
chr16:93638331-93641081
Splice length:
2747
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTACATTGTTGTTTCATTATGTCTCTTAATTGTAATTTTAGTTGACTTCAGAAAAATAATATGCAATTATGCCTTTTCAAAGTGTATCCTGAGTAACAGTGTGCATTCCTACTTAGACGTCGTTTAAGTTGCTTTCAGGTGGATTACTCCTCAGCCTTTCCTGTATTACTCAAGTGCTTCATTGCTTTTAGCACTTAATTTGCTAAGTTGACATAGCACTTACGGTGAAAATATTTATATTCTTATGAAAGTAAAAAAATAAGAATTACACTTTCCAGTACTGTGTGTACCATCCAGAGAGGTTTCATCTTTCAGGTGGTATTGCATTTATGACAGTTACTTCTTGATCCCTCTAAGGGAATGAGTATACCTTAATTATCTACTGAGCATGATAAGGTAAGGTACGTGCAGATTCGTATTAGGAGGTCATGATTTTCTTCAATTTAGGTCTATGTTTTAAAAGGGGATTTGTTTAAAAAGATTTATTTATTATGTATACAATGTTCTGCCTGGAGAGAGAGAGAGAGAGACAGAGACAGAGACAGAGACAGACAGACACACCGACTGACTGACTGACTAAGTGAGAATGGGAGAATGGTGTGGACTTTTTTCAACCTCAAAGCTCACCCCAGAAGAGGGCCAGAAGAGAGCACAAGATCTCATTATAGATGGATACGAGCCACTGTGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAATAGCCAATGTTCTTAACCTCTGAACTATCTCCAGCCCAAAGGGGGAAATTTGATTCTCAACGAATTTCCATATATATATATATATATATATATTTTTAAAGATTTATTTATTATTATATGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGAGTCAGATCTTGTTACGGATGGTTATGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGAGCAGTCAGGTGCTCTTACCCACTGAGCCATCTCACCAGCGCCCCCCCCCCCCCCCCGTATATATTTTTAAATGAGTCTTAGTTTTTTGAGTTTTTTGAGTCAGCACTAGCAAACATTCGGGTGATTTCAATTTTTCTTCTCCCAAATCTACAATGGAAGTGCTATGACCCTACAGGTATTCTCATATTTCATTGTGTTTGTTGGCAGCCGAGCAGAGATTCCCATGCCTAACAGTGGTTTTCCTTATGGTATTACCAAGCTTCAGTTTCTTTTTTCTTTTTAAAATTGGTTTATTTATTTTTTTATTTTATGTGCACTGTTTTGCCTGCATGGATGTCTCTAAGAGAATGTTGGATCCTCTGGAACTGGAGTTAGAGATAGTTGTGAGCTGCCATGTAGATGCTGGGAATTGAATCCCGGGTTCTCTGAAAGAGCATCTAATGCTCTTGACTGCCAGCCTTCTCTCCTGCCCCCAACTTAAGTTCTTTTTTCTTTTTCTTTTTCTTTTCCTTTTCCTTTTGGTTTTTTGTTTTTTGTTTTTTTGAGACAGGGTTTCTCTGTGTAGCTCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTCTGCCTTCCGAGTGCTGGGATTAAAGGCGTGTGCTACCACGCCTGGCCCCAACTTAAGTTCTTAATGAAATACTTTTTATCATTTCTGAAGTGGTTAGAAAAGATTAATTGTATGTTAATTTACAATTTAACTTATTTCTAATAAATATTTCAAAGTATGTTTCAATTAAGGGCTATTCAGATGGATCTTGAATAAATCACAAGTTGATTTTATTATTACTGATAACAGTTAATTTTTTTTTTCATTTTTTTAAAAAGATTTATTTATTTTATCTATGAGTACGCTGTTGCTGTCCTCTGACACACCAGAAGAGGGCATCAGATCCCATTATAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCACTTAGTGATCTTAACCACTGAGCCATCTCTCCAGCCCTTTTTCTTTCTATTTTTTTTAAAGATTTATTTATAATTATACATAAGTACACTGTAGCTGTCTTCAGATGCACCAGAAGAGGGCGTCAGATCTTATTAGGGGTGGTTGTGAGCCACCTTGTGGTTGCTGGGATTTGAACTCAGGACCTTTGGAAGAGCAGTCACTGAGTGCTCTTACCTGCTGAGCCATCTCACCAGCCCTCTCCAGCCCTTTTTCATTTTTAAATATATTCTTTTATGCCTGTTTGGAGAGAAATGAGTGTCTAAACAAGGATGTTAAAGGTGTTTGTTAGATGAGAAGAGTGTAGTGATAGAGTCGTAAGCTGGAAGGACCTGACAAGGATCACAGAGATATTTGTAGGTGGTGGATCTGTGTGTGTGGCTCAGAGGAGAGTGATACTGCCTTCTCTTTTCTTTATTAGCTGTTATTTAGCTTCTGGGTCAAGTGGTAGAGATCTGACACTCAGTTCTCACTTTCCCTTACAGACGGGCTTGTCTGTAGTCACCATTGTGAATGTGAGTGAGTAGTGGGTTTCACACTTGTGTGTGGTGGGTGCCAGTCTTTTCTCCTAGGAAATGAAGGCAGTTTATTCTCTTGTTTTCAAACCTTAGGAATTCCTAACCATTAGAGCTTTTAAAGTGGTAATGCTGTTCTGTTGCTTGTTACGCATTGTTCTAATTACAGTGGCTTTAAATGCAGGCTCAGAATTTCTCAAAACCCTGTTTTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTGGCTTTAAATGCAGGC
59.73
TCAGGATACACTTTGAAAAGGCA
58.28
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTGGCTTTAAATGCAGGC
59.73
CTCAGGATACACTTTGAAAAGGCA
59.24
144
PCR template sequence:
GAGCTTTTAAAGTGGTAATGCTGTTCTGTTGCTTGTTACGCATTGTTCTAATTACAGTGGCTTTAAATGCAGGCTCAGAATTTCTCAAAACCCTGTTTTCGTATGTACATTGTTGTTTCATTATGTCTCTTAATTGTAATTTTAGTTGACTTCAGAAAAATAATATGCAATTATGCCTTTTCAAAGTGTATCCTGAGTAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
26
Interacting miRNAs (#binding sites)
mmu-miR-138-5p(2), mmu-miR-139-5p(2), mmu-miR-495-3p(2), mmu-miR-296-3p(2), mmu-miR-23b-3p(1), mmu-miR-134-5p(1), mmu-miR-101a-3p(1), mmu-miR-205-5p(1), mmu-miR-335-5p(1), mmu-miR-146a-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-328-3p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-185-5p(1), mmu-miR-339-5p(1), mmu-miR-181b-5p(1), mmu-miR-214-3p(1), mmu-miR-24-3p(1), mmu-miR-494-3p(1), mmu-miR-511-3p(1), mmu-miR-3065-5p(1), mmu-miR-146b-5p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAACCCTGTTTTCGTATGTACATTGT
siRNA sets
AATGTACATACGAAAACAG, ATGTACATACGAAAACAGG, TGTACATACGAAAACAGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIMORC3_2747_ORF_3
0.4874
-0.330995408
0.009682344
165
ORF sequence:
ATGCAGGCTCAGAATTTCTCAAAACCCTGTTTTCGTATGTACATTGTTGTTTCATTATGTCTCTTAATTGTAATTTTAGTTGACTTCAGAAAAATAATATGCAATTATGCCTTTTCAAAGTGTATCCTGAGTAACAGTGTGCATTCCTACTTAGACGTCGTTTAA
Peptide sequence:
MQAQNFSKPCFRMYIVVSLCLLIVILVDFRKIICNYAFSKCILSNSVHSYLDVV
IRESfinder result
IRESfinder score
0.912454077
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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