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mmu_ciMib1_340
Basic information of circular RNA
PanCircBase ID:
mmu_ciMib1_340
mm39_Chr. Position ID:
chr18|10812156|10812496|+
Gene name:
Mib1
Transcript:
ENSMUST00000124288.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
340
Exon/intron sizes in nt:
340
Flanking exon/introns:
chr18:10812156-10817818
Splice length:
340
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTGATTGTGAGATCTTAGTATGTTCTAAAACTAGTTGGGAGTTAATCTAATGAGTTAATTTGTAATTTCATAATTTCTTTTTTTATTTTATTTTATTTTTTTTAGACTGTAATTAGACTATATATGGACCACCATCACAACGAAAACATACAGTGTTTGAACTTGCCCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTTCCTCTTGAATTTGGAACATCAAATCCATGAAAACTCATAGAAAATTTGCTTTTGGCTTTGAAGGGGAAAATCCTTTAAGGACTGCCTTTCACATTTTTCTTTTTTAATATGTGCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AATTTGCTTTTGGCTTTGAAGGG
59.12
ACAAATTAACTCATTAGATTAACTCCC
55.97
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCATAGAAAATTTGCTTTTGGCT
58.24
AACTCATTAGATTAACTCCCAACT
55.52
143
PCR template sequence:
ACATCAAATCCATGAAAACTCATAGAAAATTTGCTTTTGGCTTTGAAGGGGAAAATCCTTTAAGGACTGCCTTTCACATTTTTCTTTTTTAATATGTGCAGTATGTGATTGTGAGATCTTAGTATGTTCTAAAACTAGTTGGGAGTTAATCTAATGAGTTAATTTGTAATTTCATAATTTCTTTTTTTATTTTATTTTAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-466d-3p(3), mmu-miR-466b-3p(3), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-297a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTAATATGTGCAGTATGTGATTGTG
siRNA sets
AATCACATACTGCACATAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.97193631
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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