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mmu_ciMettl22_464
Basic information of circular RNA
PanCircBase ID:
mmu_ciMettl22_464
mm39_Chr. Position ID:
chr16|8302197|8302661|+
Gene name:
Mettl22
Transcript:
ENSMUST00000046470.16
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
464
Exon/intron sizes in nt:
464
Flanking exon/introns:
chr16:8302197-8302683
Splice length:
464
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGGGCCAGCTGCAGAGGGGTGGGGTCAGGTGGGAGTCACCCAGCGGGCCCAGTGTTGGTGGTGGCTTCTCTTTGCATCAGATGAAGGAGAGATGCCAGGTGTTCCCTTGCTGTAGCAGTTTCTGAGCATAGATGACCTGCCCTGACACCGTGACGCCTTCCTGACATCCCTGGGCTGACGGACAAGTTCTTTCCCTCATGCATTTGGCTCATCCTCTTAGCACTACCATAAGCATGCAGTCTTAAGTCATCCACCAAGCTGCCATCCCCTGCTAGGGTTATCAGTCCTGTGTGTTATTTCTAGAATCACTGTCATTGGGCACTGTGAACATGTAGGAAGAGAAGGGGGTATGGGAGAGAGTGCTGACAGAGGAACTGAGGAGATAGATAGGTGCCTGCTTAGATCATGAGGGTGGGTCGAGGAGTCAGGGTGTTTCTATGCCCATTTGTGTGCAGTCTGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGATCATGAGGGTGGGTCGA
60.03
TGCAAAGAGAAGCCACCACC
60.82
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATCATGAGGGTGGGTCGA
60.03
GATGCAAAGAGAAGCCACCAC
59.8
142
PCR template sequence:
GCTGACAGAGGAACTGAGGAGATAGATAGGTGCCTGCTTAGATCATGAGGGTGGGTCGAGGAGTCAGGGTGTTTCTATGCCCATTTGTGTGCAGTCTGGGGTGAGGGCCAGCTGCAGAGGGGTGGGGTCAGGTGGGAGTCACCCAGCGGGCCCAGTGTTGGTGGTGGCTTCTCTTTGCATCAGATGAAGGAGAGATGCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-499-5p(1), mmu-miR-300-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTGCAGTCTGGGGTGAGGGCCAGCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIMETTL22_464_ORF_1
0.7015
-0.168993179
0.020832188
111
ORF sequence:
ATGCCCATTTGTGTGCAGTCTGGGGTGAGGGCCAGCTGCAGAGGGGTGGGGTCAGGTGGGAGTCACCCAGCGGGCCCAGTGTTGGTGGTGGCTTCTCTTTGCATCAGATGA
Peptide sequence:
MPICVQSGVRASCRGVGSGGSHPAGPVLVVASLCIR
IRESfinder result
IRESfinder score
0.540665545
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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