top of page
mmu_ciMap3k2_1392
Basic information of circular RNA
PanCircBase ID:
mmu_ciMap3k2_1392
mm39_Chr. Position ID:
chr18|32359782|32361174|+
Gene name:
Map3k2
Transcript:
ENSMUST00000096575.5
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
16
Exon/intron offsets:
0
Genomic length:
1392
Exon/intron sizes in nt:
1392
Flanking exon/introns:
chr18:32359782-32361175
Splice length:
1392
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTTTGGACATCAGAGTCACCTGGAAAGTTACCTTATTTTCCTCCACATTTAAAATTGCCAGTTAAATTATCCTTTGTACCTTGGAAGTAGAATATATGAATTGTAAGTATTAATGCCATTTCAGTTATACCTTGTCAAGGCAAACTTGACTAAATATTTGAGCCTTTGAACCTATTTTTCCTTCTTTGGAAGGATTTTGTCTTAAGCAAATCTCAACATTAAAAAGCTCTTATTTCCCTGAATAACTTTTAAAGTACTCTTGGTTATCAGTGTCTTAAAAAAATACAAATCATAAAATGATGCATGATTTTTTTTCTAAACTTCAAAGGTATGTATAAACTTTTGAAACAGATAACAGCTACCTGTACGATGGTTGAACTTAGAAATAATGACTGGGGTGAAAATGGGTTCTGAGATCACCCGAGGAGATTACCTCACCTCCTGCCCACTCCTGCCCAGCCTAACACAGAGCTTGGCTCCTCACACTGATATGGGACTTCCCACTATGCGGGGTATTTACTTACGGATTTTAAAACACACAGTGAAGCGATGCAGTTCTGATAGGTCTTCATGTAATTTCTGTTTATGTGTTTGAAGAACAAGGACATTTCCATCAATAGCTGAGTTATGTTTTGATTGCACCATCAGATTTTCCAAGGATTTTGAATGTCAGCTCATATAATTATTTATAATAAATTGATGATTGCTTTACAGAAATAAAAACTGTTGCTGTGGTGGTGATTTGATGTGGTATTTTCATAATATGCTTAGCTCTGTTGTTTTTGGAAAATACATTTTATTGGATTCTGCATGATTTAAGGTATCTGCGAGTGTCCCAGGTAAGGAAGAAGAGAAATTATGCTGGTGACTATACCCTGGCAGGGCTGTGTCATGGCAGGGCTGTGTCATGGCAGGGCTGTGCGTTTGTTTAGTTATTAGTGCCTTAAAATGGAATTGATAAATTACATAATAGTTTTTAGTTAGATCATAAAATAGCCATTTCCTACTTCCCAATCAGAAAAGAAAATACACATTTTAGGAACTCAAAGGCAAATAATTTATAATGCTTGTATTCTTTGTAGGATGGAGACACAATTGTTAATGTTAGAAAATGATCGAAAACAATAAAAGTTTTTTGCCTAACCCTATACTTATGATCATAGATAATTATGATATATTTTCATTGAAATGTTTATAGCTTCAGCCTTTCATTGATATTCATGTTTGAATTACAGTTTTATTTGATTAACCTTTGAGTGAAACTTTATATTTGTAGTAAATAATATTGAAATATATGTGGACTTCATGCCAGCTTCTCATTTTTCTGGCTAACCAGATTTCTTTCCTGAAGATACTGTTTGGGTAATGGTCCTTTTTATTTTGTTTTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGGACTTCATGCCAGCTT
59.89
ACTGGCAATTTTAAATGTGGAGGA
59.16
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGTGGACTTCATGCCAGCT
59.67
ACTGGCAATTTTAAATGTGGAGGA
59.16
160
PCR template sequence:
AAATATATGTGGACTTCATGCCAGCTTCTCATTTTTCTGGCTAACCAGATTTCTTTCCTGAAGATACTGTTTGGGTAATGGTCCTTTTTATTTTGTTTTAGTAAGTTTGGACATCAGAGTCACCTGGAAAGTTACCTTATTTTCCTCCACATTTAAAATTGCCAGTTAAATTATCCTTTGTACCTTGGAAGTAGAATATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-425-5p(2), mmu-miR-181a-5p(1), mmu-miR-495-3p(1), mmu-miR-182-5p(1), mmu-miR-362-5p(1), mmu-miR-98-5p(1), mmu-miR-3061-5p(1), mmu-miR-344-3p(1), mmu-miR-338-3p(1), mmu-miR-181d-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTATTTTGTTTTAGTAAGTTTGGACA
siRNA sets
AAACTTACTAAAACAAAAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.897490127
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page