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mmu_ciLhfpl4_616
Basic information of circular RNA
PanCircBase ID:
mmu_ciLhfpl4_616
mm39_Chr. Position ID:
chr6|113152790|113153406|-
Gene name:
Lhfpl4
Transcript:
ENSMUST00000162280.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
616
Exon/intron sizes in nt:
616
Flanking exon/introns:
chr6:113148919-113153406
Splice length:
616
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCAAGTGTGTGGACCAGAGGGCAAGGCCTGCTGTGAGGCAGGAAAGGGCAACCGCAGATGCAATAAAAGGAGTGAAGCAAAAATTACACACAGGGAGCTGGCGATTCAGAGCACTGGCTACTCTTCCAGAGGCCCTGGGCTGATTCCCAACATCCACGTGAAGCCACAGGGCCATCTTTAAGTCTAGTTCTAGAGATTCAACGTCCTCTTCTGGCCTCCTCTGGCACTGTCCACATAGTGCACATAAATACACTCAAGCAAAATCACCCATACACTTAAACGATTTTAAAAAGGAGTACACAGAGGCCATTAAGATGGCTCAACAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCTTAATGCAATCTGATGCCCTCTTCTGGAGTATCTGAGGACAGCTACAGTGTACTTATATATAATAAATAAATAAATCTTAAAAAAAAAAAAAAAAGATGGCTCAACAGTAAAAGGCACTTGATGCTAAACTCGATGATCTGAGTTTGATCCCCAGGATCCACAAGGTGGAAAGAGACCCTGGAAAATTTGTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCTCAACAGTAAAAGGCAC
59.05
TTATTGCATCTGCGGTTGCC
59.54
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCTCAACAGTAAAAGGCA
57.64
TTATTGCATCTGCGGTTGCC
59.54
161
PCR template sequence:
AAAGATGGCTCAACAGTAAAAGGCACTTGATGCTAAACTCGATGATCTGAGTTTGATCCCCAGGATCCACAAGGTGGAAAGAGACCCTGGAAAATTTGTCGCAAGTGTGTGGACCAGAGGGCAAGGCCTGCTGTGAGGCAGGAAAGGGCAACCGCAGATGCAATAAAAGGAGTGAAGCAAAAATTACACACAGGGAGCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-29b-2-5p(1), mmu-miR-673-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGAAAATTTGTCGCAAGTGTGTGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.432199126
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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